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Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine

Fatemeh Askarian1,2, John D Lapek3, Mitesh Dongre4

  • 1Research Group of Host Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT - The Arctic University of Norway, Tromsø, Norway.

Frontiers in Microbiology
|March 9, 2018
PubMed

Insights

Staphylococcus aureus membrane-derived vesicles (MVs) influence host interactions and survival. Immunizing mice with these MVs provides protection against S. aureus infections.

Area of Science:

  • Microbiology
  • Immunology
  • Proteomics

Background:

  • Staphylococcus aureus produces membrane-derived vesicles (MVs).
  • These MVs share functional similarities with outer membrane vesicles.
  • MV release is influenced by bacterial growth conditions.

Purpose of the Study:

  • To characterize Staphylococcus aureus MVs.
  • To investigate the role of MVs in bacterial pathogenesis and host immunity.
  • To evaluate the potential of MVs as a vaccine candidate.

Main Methods:

  • Atomic force microscopy to visualize MVs.
  • Comparative proteomic analysis to identify MV proteins.
  • In vitro assays for cytotoxicity and neutrophil activation.
  • In vivo studies including bacterial survival and murine immunization models.

Main Results:

  • Proteomic analysis identified significant differences in protein content of MVs based on growth media.
  • S. aureus MVs induced comparable cytotoxicity and neutrophil activation.
  • Exogenous MVs enhanced S. aureus resistance to host defenses and survival.
  • Immunization with MVs conferred protection against S. aureus infection in mice.

Conclusions:

  • Staphylococcus aureus MVs play a role in bacterial-host interactions during infection.
  • S. aureus MVs can modulate the host immune response.
  • S. aureus MVs hold promise as a protective vaccine strategy against S. aureus infections.
Keywords:
Staphylococcus aureusmembrane-derived vesiclesprotective immunityproteomicssystemic infection

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