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TILLING: The Next Generation.

Bradley J Till1, Sneha Datta2, Joanna Jankowicz-Cieslak2

  • 1Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA Laboratories Seibersdorf, International Atomic Energy Agency, Vienna International Centre, Vienna, Austria. b.till@iaea.org.

Advances in Biochemical Engineering/Biotechnology
|March 9, 2018
PubMed
Summary
This summary is machine-generated.

TILLING, a reverse-genetics strategy, has advanced plant functional genomics by combining chemical mutagenesis with high-throughput mutation discovery. This review highlights key milestones and future directions in exploring plant genomes.

Keywords:
CRISPR/CasChemical mutagenesisEMSIn silico TILLINGNext generation sequencing

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Area of Science:

  • Plant Functional Genomics
  • Reverse Genetics

Background:

  • TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse-genetics strategy.
  • It combines high-density chemical mutagenesis with high-throughput mutation discovery.

Purpose of the Study:

  • To review the 18-year mission of TILLING in plant functional genomics.
  • To highlight key milestones, technological advancements, and future strategies.

Main Methods:

  • High-density chemical mutagenesis
  • High-throughput mutation discovery
  • Next-generation sequencing
  • In silico databases of EMS-induced mutations
  • Exome capture sequencing in wheat

Main Results:

  • Establishment of TILLING in diverse plant species.
  • Insights into the effects of chemical mutagens on plant genomes.
  • Development of advanced mutation discovery and cataloging strategies.

Conclusions:

  • TILLING has been instrumental in exploring plant gene space.
  • Future directions include targeted genome editing for reverse genetics.