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Related Experiment Videos

Sea urchin prosome: characterization and changes during development.

O Akhayat, F Grossi de Sa, A A Infante

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1987
    PubMed
    Summary

    Sea urchin eggs contain prosomes, cytoplasmic particles similar to those in duck cells. These particles remain stable during early development, with their location shifting from the cytoplasm to nuclei.

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    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Biochemistry

    Background:

    • Cytoplasmic particles, termed prosomes, are found in duck erythroblasts.
    • Similar particles have been identified in sea urchin eggs and embryos.

    Purpose of the Study:

    • To isolate and characterize sea urchin prosomes.
    • To investigate the behavior and localization of prosomes during early sea urchin development.

    Main Methods:

    • Sucrose gradient sedimentation and Cs2SO4 density gradient centrifugation for purification and physical property determination.
    • Biochemical analysis to characterize polypeptide and RNA content.
    • Monoclonal antibody against duck prosome protein used for immunoblotting and immunofluorescence.
    • Cellular fractionation and in vivo labeling experiments.

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    Main Results:

    • Sea urchin prosomes sediment between 16-19S with a buoyant density of 1.30 g/cm³.
    • Particles consist of 20-25 polypeptides (24-35 kDa) and small RNAs.
    • A 27-kDa polypeptide is conserved between duck and sea urchin prosomes.
    • Prosome levels remain constant, but subcellular localization shifts from postribosomal supernatant to nuclear fractions during development.
    • No de novo synthesis detected in early development, indicating metabolic stability.

    Conclusions:

    • Sea urchin prosomes are metabolically stable and likely synthesized during oogenesis.
    • Their subcellular localization changes significantly during early embryogenesis.
    • Prosomess are normal cellular constituents in sea urchins.