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Inhibitor of apoptosis proteins, NAIP, cIAP1 and cIAP2 expression during macrophage differentiation and M1/M2

Virginia Morón-Calvente1,2, Salvador Romero-Pinedo2,3, Sofía Toribio-Castelló2

  • 1Department of Cell Biology, University of Granada, Granada, Spain.

Plos One
|March 9, 2018
PubMed

Insights

This study reveals how Inhibitor of Apoptosis (IAP) proteins, including NAIP, cIAP1, and cIAP2, change during macrophage polarization. These findings offer insights into immune cell regulation and potential therapeutic targets.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Monocytes and macrophages are crucial immune cells with plastic phenotypes (M1 and M2) for defense and repair.
  • Inhibitor of Apoptosis (IAP) proteins regulate cellular processes, including immunity.

Purpose of the Study:

  • To investigate the differential expression of NAIP, cIAP1, and cIAP2 during macrophage differentiation and polarization.
  • To analyze the impact of the IAP antagonist SMC-LCL161 on IAP expression in polarized macrophages.

Main Methods:

  • Analysis of NAIP, cIAP1, and cIAP2 expression in polarized THP-1 cells and primary human macrophages.
  • Treatment of macrophages with the IAP antagonist SMC-LCL161.

Main Results:

  • NAIP is highly expressed in M2 macrophages, while cIAP1 and cIAP2 show distinct expression patterns in M1 and M2 phenotypes.
  • SMC-LCL161 treatment modulated NAIP, cIAP1, and cIAP2 expression, with NAIP upregulated in M2, cIAP1 downregulated in both M1/M2, and cIAP2 induced in M1.

Conclusions:

  • IAP protein expression is differentially regulated during macrophage polarization.
  • IAP antagonists can modulate macrophage polarization, suggesting potential therapeutic applications in immune modulation.

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