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Updated: Feb 13, 2026

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
Published on: December 16, 2016
Characterization of microplastic litter from oceans by an innovative approach based on hyperspectral imaging
Silvia Serranti1, Roberta Palmieri1, Giuseppe Bonifazi1
1Department of Chemical Engineering, Materials and Environment, Sapienza University of Rome, Via Eudossiana 18, 00184 Rome, Italy.
Abstract:
An innovative approach, based on HyperSpectral Imaging (HSI), was developed in order to set up an efficient method to analyze marine microplastic litter. HSI was applied to samples collected by surface-trawling plankton nets from several parts of the world (i.e. Arctic, Mediterranean, South Atlantic and North Pacific). Reliable information on abundance, size, shape and polymer type for the whole ensemble of plastic particles in each sample was retrieved from single hyperspectral images. The simultaneous characterization of the polymeric composition of the plastic debris represents an important analytical advantage considering that this information, and even the validation of the plastic nature of the small debris, is a common flaw in the analysis of marine microplastic pollution. HSI was revealed as a rapid, non-invasive, non-destructive and reliable technology for the characterization of the microplastic waste, opening a promising way for improving the plastic pollution monitoring.
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