[Lymphocyte migration induced by orciprenaline using the Boyden technic]

The influence of the beta-sympathomimetic drug orciprenaline on the lymphocyte migration was investigated using the Boyden-technique. Orciprenaline was able to stimulate the migration of lymphocytes derived from lymph nodes of rats in a concentration range of 10(-10) to 10(-6) M. After 4.5 hours the maximum of the stimulation was reached. The supernatants collected after the stimulation of the lymph node cells with orciprenaline were also able to enhance the migration of PMN derived from guinea pigs of other lymphocyte cultures. The orciprenaline-induced stimulation of the lymphocyte migration was inhibited by propranolol (10(-9) M), whereas the addition of propranolol to the orciprenaline-evoked supernatants did not antagonize its stimulatory activity on the lymphocyte migration. After the concentration and the fractionation (Ultrogel AcA54) of the supernatants the migration stimulating activity could be demonstrated at the molecular weights of 40, 24, 18 and smaller than 12 kDa. It is supposed that sympathomimetic drugs are capable of inducing the lymph node cells to secrete mediators, which are responsible for the stimulation of the lymphocyte migration.