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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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RNA Editing02:23

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

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Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Related Experiment Video

Updated: Feb 13, 2026

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
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Inducible Genome Editing with Conditional CRISPR/Cas9 Mice.

Alexandra Katigbak1, Francis Robert1, Marilène Paquet2

  • 1Department of Biochemistry, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.

G3 (Bethesda, Md.)
|March 10, 2018
PubMed
Summary

Researchers developed a new transgenic mouse model for inducible genome engineering. This flexible platform uses doxycycline-regulated Cas9 and exogenous sgRNAs for precise gene editing in vivo.

Keywords:
CRISPR/Cas9Cas9 knock-inconditional Cas9 mousegenome editingmouse model

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Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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Area of Science:

  • * Genetics and Genomics
  • * Molecular Biology
  • * Animal Models

Background:

  • * Genetically engineered mouse models (GEMMs) are crucial for studying gene function, disease mechanisms, and drug responses.
  • * Advanced GEMMs allow for tissue-specific and inducible gene manipulation.
  • * Existing models have limitations in temporal and spatial control of genetic modifications.

Purpose of the Study:

  • * To create a versatile transgenic mouse model for inducible and precise genome engineering.
  • * To establish a platform for studying gene function and disease pathogenesis.
  • * To facilitate the identification of genetic modifiers influencing biological processes and drug responses.

Main Methods:

  • * Generation of a transgenic mouse line expressing a doxycycline-regulated Cas9 allele.
  • * Utilizing exogenous delivery of single-guide RNAs (sgRNAs) for targeted genome editing.
  • * Implementing a Tet-On/Tet-Off system for temporal control of Cas9 activity.

Main Results:

  • * Successful generation of a transgenic mouse model with inducible Cas9 expression.
  • * Demonstration of efficient and targeted genome editing upon doxycycline induction.
  • * Validation of the model's flexibility for various genome engineering applications.

Conclusions:

  • * The developed doxycycline-inducible Cas9 mouse model offers a powerful and flexible tool for in vivo genome engineering.
  • * This model enables precise temporal and spatial control over gene editing, advancing the study of gene function and disease.
  • * It provides a valuable platform for creating sophisticated models of biological and pathological processes.