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The anticancer activity of antisense microRNA (fRNA) in combination with the lectin from Bacillus subtilis B-7025
Artur Martynov1, Gennady Didenko2, Boris Farber3
1SI 'Mechnikov Institute of Microbiology and Immunology, National Academy of Medical Sciences of Ukraine', Kharkov, Ukraine.
Objectives:
Many adenocarcinomas have the ability to capture from an extracellular matrix the oligonucleotides and nanoparticles by pinocytosis, when the non-cancerous cells are not capable to capture the oligonucleotides and small liposomes. This provides selective accumulation of proposed protected oligonucleotides (fRNA) in cancer cells and also provides the absence toxicity in the fRNA.
Methods:
For the immunotherapy, we used immunotropic 70 kDa lectin Bacillus subtilis B-7025. In vivo experiments were carried out in C57BL line mice in Lewis lung carcinoma. The cytotoxic activity, lymphocytes and macrophages were determined in vitro using the MTT assay.
Key Findings:
Animal survival rate in groups receiving either the fRNA or lectine was 70 and 40%, respectively.
Conclusions:
Combined use of fRNA and lectine has the advantage compared with the use of these drugs in monotherapy, as the anticancer efficacy of the scheme is much higher, which is manifested in the primary tumour node and metastasis inhibition.
Insights
Protected oligonucleotides (fRNA) selectively accumulate in cancer cells via pinocytosis, offering a novel cancer therapy. Combined fRNA and lectin treatment significantly inhibits tumor growth and metastasis, improving survival rates.
Area of Science:
- Oncology
- Immunotherapy
- Nanomedicine
Background:
- Adenocarcinomas exhibit unique pinocytosis for extracellular oligonucleotides and nanoparticles, unlike normal cells.
- This selective uptake facilitates targeted delivery of therapeutic agents to cancer cells.
- Protected oligonucleotides (fRNA) demonstrate selective accumulation in tumor cells with no observed toxicity.
Purpose of the Study:
- To evaluate the efficacy of protected oligonucleotides (fRNA) as a targeted cancer therapy.
- To investigate the combined therapeutic effect of fRNA and Bacillus subtilis B-7025 lectin in a Lewis lung carcinoma model.
- To assess the impact of this combined therapy on animal survival and tumor progression.
Main Methods:
- In vivo studies were conducted using C57BL mice bearing Lewis lung carcinoma.
- Immunotherapy involved the use of Bacillus subtilis B-7025 lectin.
- Cytotoxic activity of lymphocytes and macrophages was determined using the MTT assay in vitro.
Main Results:
- Animal survival rates were 70% for fRNA monotherapy and 40% for lectin monotherapy.
- Combined fRNA and lectin therapy demonstrated superior anticancer efficacy compared to monotherapy.
- The combination treatment significantly inhibited primary tumor growth and metastasis.
Conclusions:
- Combined fRNA and lectin therapy offers significant advantages over monotherapy for treating Lewis lung carcinoma.
- This combined approach enhances anticancer efficacy through synergistic effects.
- The strategy shows promise for inhibiting primary tumor development and preventing metastasis.
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