Dynamic Sparse Sampling for Confocal Raman Microscopy
Related Experiment Video
Updated: Feb 13, 2026

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
Published on: September 9, 2022
Shijie Zhang1, Zhengtian Song1, G M Dilshan P Godaliyadda2
1Department of Chemistry , Purdue University , West Lafayette , Indiana 47907 , United States.
Abstract:
The total number of data points required for image generation in Raman microscopy was greatly reduced using sparse sampling strategies, in which the preceding set of measurements informed the next most information-rich sampling location. Using this approach, chemical images of pharmaceutical materials were obtained with >99% accuracy from 15.8% sampling, representing an ∼6-fold reduction in measurement time relative to full field of view rastering with comparable image quality. This supervised learning approach to dynamic sampling (SLADS) has the distinct advantage of being directly compatible with standard confocal Raman instrumentation. Furthermore, SLADS is not limited to Raman imaging, potentially providing time-savings in image reconstruction whenever the single-pixel measurement time is the limiting factor in image generation.
More Related Videos
Related Concept Videos
Confocal Fluorescence Microscopy
Preparation of Samples for Electron Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
Dynamic Equilibrium
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

