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Updated: Feb 13, 2026

Translational Orthotopic Models of Glioblastoma Multiforme
Published on: February 17, 2023
MGMT pyrosequencing-based cut-off methylation level and clinical outcome in patients with glioblastoma multiforme
Lorena Gurrieri1,2, Elisa De Carlo1,3, Lorenzo Gerratana1,4
1Department of Oncology, University Hospital of Udine, Udine 33100, Italy.
Aim:
MGMT promoter methylation has been associated with improved survival in glioblastoma multiforme treated with temozolomide. However, there is no consensus on specific cut-off levels of methylation. The aims of the study were to explore the prognostic impact of MGMT methylation status and to analyze the role of specific cut-off values.
Materials & Methods:
We analyzed 108 glioblastoma multiforme patients treated between 2008 and 2013 stratified according to three pyrosequencing-based quantitative methylation in: unmethylated (methylation <9%), intermediate (9-29%) and highly methylated (>29%).
Results:
The three-class stratification has a prognostic impact (median progression-free survival: 7.97, 11.6 and 15 months respectively; p = 0.004; median OS: 13.2, 15.8 and 19.5 months, respectively; p = 0.0002), especially in patients exposed to temozolomide.
Conclusion:
Our study confirmed that the independent prognostic role of MGMT methylation status. An average level of methylation between all investigated CpGs of 9% may help discriminating between methylated and unmethylated tumors.
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