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Tenacibaculum todarodis sp. nov., isolated from a squid
Su-Kyoung Shin1, Eunji Kim1, Hana Yi2,3,1
1Institute for Biomaterials, Korea University, Seoul, Republic of Korea.
Summary
A novel aerobic bacterium, Tenacibaculum todarodis sp. nov., was isolated from squid. This Gram-negative bacterium represents a new species within the Tenacibaculum genus, distinguished by unique genomic and biochemical traits.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Genomics
Background:
- The genus Tenacibaculum comprises marine bacteria with diverse ecological roles.
- Accurate taxonomic classification is crucial for understanding microbial diversity and function in marine ecosystems.
Purpose of the Study:
- To characterize a novel bacterial strain isolated from the squid Todarodes pacificus.
- To determine the phylogenetic and chemotaxonomic relationship of the isolate to existing Tenacibaculum species.
- To formally propose a new species within the genus Tenacibaculum.
Main Methods:
- Isolation and cultivation of bacterial strain LPB0136 from squid.
- Genomic analysis including chromosome size, G+C content, and gene prediction.
- Phylogenetic analysis using 16S rRNA gene sequences.
- Chemotaxonomic analysis (respiratory quinone, polar lipids, fatty acids).
- Biochemical and physiological characterization.
Main Results:
- Strain LPB0136 is a Gram-negative, rod-shaped, aerobic bacterium.
- Genomic analysis revealed a 3.02 Mb chromosome with 30.7% G+C content and 2726 protein-coding genes.
- Phylogenetic analysis placed the isolate within the genus Tenacibaculum, distinct from known species with 95.9% 16S rRNA gene similarity to T. aestuarii and T. caenipelagi.
- Chemotaxonomic and fatty acid profiles were consistent with the genus but showed quantitative differences.
- Biochemical and physiological tests further distinguished the isolate from related species.
Conclusions:
- Strain LPB0136 represents a novel species of the genus Tenacibaculum.
- The proposed name for this new species is Tenacibaculum todarodis sp. nov.
- This discovery expands the known diversity of the Tenacibaculum genus in marine environments.
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