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    Acousto-optic devices in optical tweezers have positioning inaccuracies. A new method randomizes radio frequency signal phases, eliminating these errors without adding noise, improving high-resolution applications.

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    Area of Science:

    • Physics
    • Optical Engineering
    • Biophysics

    Background:

    • Acousto-optic (AO) devices offer flexibility and speed in optical tweezers.
    • Existing AO devices suffer from trap positioning inaccuracies, limiting high-resolution applications.

    Purpose of the Study:

    • To identify the cause of inaccuracies in AO device-based optical tweezers.
    • To develop and validate a method for eliminating these inaccuracies.

    Main Methods:

    • Investigated interference patterns within AO device sound fields as the source of inaccuracies.
    • Developed a method to reduce sound field coherence by randomizing the phase of the radio frequency voltage input signal.
    • Tested the method on both acousto-optic modulator and deflector devices.

    Main Results:

    • Interference patterns in AO sound fields were confirmed as the cause of trap positioning inaccuracies.
    • The random phase method successfully eliminated trapping inaccuracies in both constant trap position and force-ramp measurements.
    • No additional noise was introduced by the implemented method.

    Conclusions:

    • The random phase method effectively removes inaccuracies in AO device-based optical tweezers.
    • This technique is applicable to various AO device types and easily integrated into existing systems.
    • The findings significantly enhance the utility of optical tweezers for high-resolution applications.