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Updated: Feb 13, 2026

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae
Published on: June 25, 2018
Thermal Requirements and Development Response to Constant Temperatures by Nesidiocoris tenuis (Hemiptera: Miridae),
Mohammad Ali Mirhosseini1, Yaghoub Fathipour1, Mahmoud Soufbaf2
1Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Abstract:
Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae), a common zoophytophagus bug, is a biological control agent for several groups of noxious agricultural pests, including whiteflies, aphids, and leafminers. To improve mass-rearing of this species and to optimize its application in integrated pest management, nymphal survival and developmental times of this predator were examined at seven constant temperatures ranging from 14 through 34°C. Eggs developed to adulthood at all temperatures tested. Egg-adult developmental time decreased sharply with increasing temperature, except at 34°C (17.21 d), for which developmental time was significantly longer than that obtained at 31°C (15.59 d). The lowest (11.36%) and highest (28.26%) percentage of mortality was found at 28 and 14°C, respectively. To describe the development rate of immature stages of N. tenuis as a function of temperature, two linear and 26 nonlinear models were fitted. The lower temperature threshold (T0) and thermal constant (K) of total immature stages were estimated by the ordinary (10.94°C and 318.37 DD) and Ikemoto (10.28°C and 339.57 DD) linear models. Based on the Akaike information criterion (AIC), Lactin-1, Analytis-1/Allahyari and Janisch/Kontodimas were the best models to describe the temperature-dependent development rate of egg, nymph and whole immature stages of the predator, respectively. Our findings provide information on N. tenuis biology that will improve application of this predator as a biological control agent.
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