Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

98.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.9K
Generation Time01:22

Generation Time

1.6K
Bacterial generation time, the period required for a bacterial population to double during its exponential growth phase, serves as a critical measure of microbial growth dynamics under optimal conditions. This parameter varies significantly across bacterial species and can be influenced by factors such as temperature, pH, and the availability of nutrients. For example, Escherichia coli can achieve a generation time of approximately 20 minutes, while Mycobacterium tuberculosis exhibits a much...
1.6K
Real Time RT-PCR02:57

Real Time RT-PCR

65.4K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.4K
Passive Filters01:27

Passive Filters

1.0K
Passive filters are utilized to shape the frequency spectrum of signals across a diverse array of applications. These filters, using only passive elements like resistors (R), inductors (L), and capacitors (C), are capable of selectively allowing or blocking certain frequency ranges without the need for external power sources.
Low-Pass Filters
Low-pass filters are designed to transmit signals with frequencies lower than the cutoff frequency, ωc, and attenuate those above it. The cutoff...
1.0K
Active Filters01:25

Active Filters

1.4K
Active filters are electronic circuits that use operational amplifiers (op-amps), resistors, and capacitors to filter out unwanted frequency components from a signal. A first-order low-pass active filter is designed to pass signals with a frequency lower than a certain cutoff frequency and attenuate frequencies higher than that cutoff frequency. The transfer function for a first-order low-pass active filter is:
1.4K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Foundation model enables interpretable open and error-tolerant searching for mass spectrometry-based proteomics.

Bioinformatics (Oxford, England)·2026
Same author

Nipah Virus Shedding in Urine from Fruit Bats, Sri Lanka, 2018-2019.

Emerging infectious diseases·2026
Same author

Dengue NS1 as a Driver of Immune-Mediated Pathogenesis.

Tropical medicine and infectious disease·2026
Same author

Monitoring viral evolution and epidemiological characteristics of SARS-CoV-2 during 2022-2023 using Integrated Genomic Surveillance.

Communications medicine·2026
Same author

Selecting synthetic data for successful simulation-based transfer learning in dynamical biological systems.

BMC bioinformatics·2026
Same author

Skin and Wound Infection Models to Test Host-Pathogen Interactions and Evaluate Topical Antimicrobial Treatments.

Methods in molecular biology (Clifton, N.J.)·2026

Related Experiment Video

Updated: Feb 13, 2026

Real-Time fMRI Brain Mapping in Animals
04:05

Real-Time fMRI Brain Mapping in Animals

Published on: September 24, 2020

4.1K

PriLive: privacy-preserving real-time filtering for next-generation sequencing.

Tobias P Loka1, Simon H Tausch1,2, Piotr W Dabrowski1

  • 1Bioinformatics Division (MF 1), Department for Methods Development and Research Infrastructure.

Bioinformatics (Oxford, England)
|March 10, 2018
PubMed
Summary

PriLive is a new tool that removes sensitive human DNA data during next-generation sequencing. This real-time filtering enhances data privacy and speeds up analysis by reducing data volume.

More Related Videos

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.0K
Real-time Tracking of DNA Fragment Separation by Smartphone
06:58

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

15.3K

Related Experiment Videos

Last Updated: Feb 13, 2026

Real-Time fMRI Brain Mapping in Animals
04:05

Real-Time fMRI Brain Mapping in Animals

Published on: September 24, 2020

4.1K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.0K
Real-time Tracking of DNA Fragment Separation by Smartphone
06:58

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

15.3K

Area of Science:

  • Genomics
  • Bioinformatics
  • Data Privacy

Background:

  • Next-generation sequencing (NGS) data, even anonymized, poses re-identification risks.
  • Current data protection methods are applied post-sequencing, causing delays and insufficient protection.
  • Human DNA in byproduct samples (viral, metagenomic) requires robust privacy measures.

Purpose of the Study:

  • To introduce PriLive, a novel tool for real-time removal of sensitive human DNA data during sequencing.
  • To enable compliance with strict data protection regulations in genomic data analysis.
  • To accelerate downstream analyses by reducing data input and processing time.

Main Methods:

  • PriLive automates the detection and removal of human sequence information during the sequencing process.
  • The tool operates in real-time, minimizing delays in the analysis workflow.
  • Performance is validated against conventional post-hoc filtering techniques.

Main Results:

  • PriLive effectively removes sensitive human DNA data during sequencing.
  • The tool achieves filtering accuracy comparable to existing post-hoc methods.
  • Real-time processing significantly accelerates subsequent analyses, especially with high background noise.

Conclusions:

  • PriLive offers a proactive solution for genomic data privacy.
  • The tool enhances data protection and analysis efficiency in NGS.
  • PriLive is an open-source solution facilitating widespread adoption and further development.