Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Affinity03:07

Electron Affinity

43.7K
The electron affinity (EA) is the energy change for adding an electron to a gaseous atom to form an anion (negative ion).
43.7K
Affinity and Avidity01:41

Affinity and Avidity

39.3K
Overview
39.3K
Adrenergic Receptors: ɑ Subtype01:31

Adrenergic Receptors: ɑ Subtype

3.0K
Adrenoceptors are classified into α and ꞵ classes based on their potencies to catecholamine agonists. α-adrenoceptors show the following order of catecholamine potency:
Adrenaline ≥ Noradrenaline >> Isoprenaline
α-adrenoceptors are further divided into α1 and α2-adrenoceptors.
α1-Adrenoceptors: These receptors are located postsynaptically on the effector organs and cause constriction of smooth muscle mediated by activation of phospholipase...
3.0K
Adrenergic Receptors: β Subtype01:26

Adrenergic Receptors: β Subtype

3.9K
β-adrenoceptors have varied sensitivities towards adrenaline, noradrenaline, and isoprenaline. The order of agonist potency is as follows:
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors...
3.9K
Affinity Chromatography01:03

Affinity Chromatography

3.1K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
3.1K
Diabetes Mellitus: Overview and Type I Subtype01:22

Diabetes Mellitus: Overview and Type I Subtype

5.5K
Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacological modulation of TRPV2 enhances migration and induces Immunoglobulin E (IgE)-independent degranulation of mast cells.

Cell calcium·2026
Same author

Concise Synthesis of (±)-Stephadiamine via Vinylogous Wenker Cyclization.

Journal of the American Chemical Society·2026
Same author

Cigarette smoke extract (CSE) reduces expression of functional TRPV4 channels in primary human bronchial epithelial cells differentiated at an air liquid interface (ALI) in vitro.

Archives of toxicology·2026
Same author

Protecting Groups as Dispersive Directing Groups: Toward the Asymmetric Synthesis of Altemicidin.

Organic letters·2026
Same author

O-linked glycan-dependent gating of TPC2 controls lysosomal excitability and organelle remodeling.

Nature communications·2026
Same author

An Agonist/Antagonist Photo-Switchable Vitamin D Mimetic Enables Bidirectional Optical Control of VDR.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Feb 13, 2026

Subtype-selective Electroporation of Cortical Interneurons
06:42

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

9.2K

A (+)-Larixol Congener with High Affinity and Subtype Selectivity toward TRPC6.

Stephanie Häfner1, Finn Burg2, Martina Kannler3

  • 1Rudolf Boehm Institute for Pharmacology and Toxicology, Leipzig University, Härtelstr. 16-18, 04107, Leipzig, Germany.

Chemmedchem
|March 10, 2018
PubMed
Summary

A new compound, SH045, selectively blocks the TRPC6 channel, offering a potential treatment for lung ischemia-reperfusion edema. This natural product derivative shows promise for improving patient outcomes after organ transplantation.

Keywords:
LIRETRPC6diterpeneslabdanelarixol

More Related Videos

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

35.1K
Laboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout Salvelinus namaycush from Its Prey
12:24

Laboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout Salvelinus namaycush from Its Prey

Published on: August 29, 2014

11.4K

Related Experiment Videos

Last Updated: Feb 13, 2026

Subtype-selective Electroporation of Cortical Interneurons
06:42

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

9.2K
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

35.1K
Laboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout Salvelinus namaycush from Its Prey
12:24

Laboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout Salvelinus namaycush from Its Prey

Published on: August 29, 2014

11.4K

Area of Science:

  • Pharmacology and Natural Products Chemistry
  • Ion Channel Physiology
  • Translational Medicine

Background:

  • Natural products offer health benefits and life quality improvements.
  • The diterpene (+)-larixol and its derivatives selectively inhibit the TRPC6 cation channel.
  • TRPC6 channels are implicated in various physiological processes and disease states.

Purpose of the Study:

  • To chemically diversify (+)-larixol to develop more potent and selective TRPC6 inhibitors.
  • To evaluate the efficacy of novel compounds in cellular and ex vivo models.
  • To explore the therapeutic potential of TRPC6 inhibition for lung ischemia-reperfusion edema (LIRE).

Main Methods:

  • High-throughput calcium (Ca2+) FLIPR screening assays.
  • Electrophysiological patch-clamp recordings in HEK293 cells and rat pulmonary smooth muscle cells.
  • Assessment of SH045 in isolated perfused lung preparations to model LIRE.

Main Results:

  • Larixyl N-methylcarbamate (SH045) demonstrated nanomolar affinity and 13-fold subtype selectivity for TRPC6 over TRPC3.
  • TRPC6 inhibition by SH045 was confirmed in rat pulmonary smooth muscle cells.
  • SH045 treatment significantly reduced lung ischemia-reperfusion edema in ex vivo lung preparations.

Conclusions:

  • SH045 is a potent and selective TRPC6 blocker derived from (+)-larixol.
  • SH045 effectively mitigates lung ischemia-reperfusion edema in a preclinical model.
  • The straightforward and scalable synthesis of SH045 supports its potential for clinical translation in treating LIRE.