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Updated: Feb 13, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Published on: September 11, 2022
A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae
Aurèle Piazza1, Romain Koszul2, Wolf-Dietrich Heyer3
1University of California, Davis, Davis, CA, United States; Institut Pasteur, Paris, France.
Abstract:
Homologous recombination faithfully restores the sequence information interrupted by a DNA double-strand break by referencing an intact DNA molecule as a template for repair DNA synthesis. DNA synthesis is primed from 3'-OH end of the invading DNA strand in the displacement loop (D-loop). Here, we describe a simple and quantitative proximity ligation-based assay to study the initiation of homologous recombination-associated DNA synthesis initiated at the D-loop and final product formation. The D-loop extension assay overcomes the semiquantitative nature and some limitations of the current PCR-based technique and facilitates the study of the recombination-associated DNA synthesis.
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