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Updated: Feb 13, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
A fluorescent sensor for fast detection of peroxynitrite by removing of C=N in a benzothiazole derivative
Youming Shen1, Xiangyang Zhang1, Youyu Zhang2
1Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde, 415000, PR China.
Abstract:
A simple structured reaction-based probe for peroxynitrite (ONOO-) was developed by using benzothiazole hydrazone base of the excited-state intramolecular proton transfer (ESIPT) dye. It was constructed from 2-hydrazinylpyridine and 3-(benzo[d]thiazol-2-yl)-2-hydroxy-5-methylbenzaldehyde which possesses itself weak fluorescence and displayed prominent fluorescence response towards ONOO- by oxidizes the C=N bond to an aldehyde in aqueous solution. The probe exhibited excellent selectivity and high sensivity towards ONOO- with a low dectection limit of 5.8 × 10-8 M in physiological condition. Moreover, the probe could realize fast recognition to ONOO- in as short as 60 s. In addition, the probe was also successfully used for living cell imaging.
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