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Updated: Feb 13, 2026
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
Published on: December 9, 2010
A resumable two-photon fluorescent probe for Cu2+ and S2- based on magnetic silica core-shell Fe3O4@SiO2
Huie Jiang1, Yan Liu2, Weifang Luo2
1Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China; College of Bioresources Chemistry and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, PR China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
Abstract:
A two-photon fluorescent probe for Cu2+ and S2- has been strategically prepared with naphthalimide derivative platform (NPE) covalently grafted onto the surface of magnetic core-shell Fe3O4@SiO2 nanoparticles. The probe (NPE-Fe3O4@SiO2) exhibits selective response to Cu2+ with enhanced fluorescence and efficient separation of Cu2+ with external magnetic field. The consequent product NPE-Fe3O4@SiO2-Cu of NPE-Fe3O4@SiO2 and Cu2+ can work as an excellent sensor for S2- by removing Cu2+ from the complex with fluorescence decreased, recovering the fluorescence of the probe. Therefore, the constituted Off-On-Off type fluorescence monitoring system means the probe is resumable. Moreover, the probe has been used to quantitatively detect Cu2+ and S2- with low detection limits, which are 0.28 μM and 0.12 μM, respectively. Furthermore, the probe shows low cytotoxicity and excellent membrane permeability, which has been successfully applied for monitoring Cu2+ and S2- in living cells and imaging Cu2+ in deep-tissue with two-photon excited fluorescence.
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