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Updated: Feb 13, 2026

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge
Published on: September 26, 2016
Qualitative and quantitative spectrometric evaluation of soluble microbial products formation in aerobic granular
Heng Dong1, Dong Wei2, Jinglin Wei1
1School of Resources and Environment, University of Jinan, Jinan, 250022, People's Republic of China.
Abstract:
In present study, the characteristics of soluble microbial products (SMP) were evaluated in aerobic granular sludge system during denitrification process under different chemical oxygen demand/nitrogen (C/N) ratios. Batch experiment showed that the effluent nitrate (NO3--N) concentration were 15.24 ± 1.83 and 1.72 ± 1.53 mg/L at C/N ratio of 1 and 6, respectively. For the release of SMP, the protein (PN) and polysaccharide contents increased from 1.23 ± 0.38 and 7.46 ± 1.13 mg/L to 1.80 ± 0.76 and 10.53 ± 1.24 mg/L with increasing C/N ratios, respectively. Excitation-emission matrix identified four peaks in SMP, including aromatic PN-like, tryptophan PN-like, fulvic acid-like and humic acid-like substances. Fluorescence regional integration suggested that biodegradable PN-like substances occupied the percentage between 53.0 and 61.7% in SMP. Synchronous fluorescence spectra coupled with two-dimensional correlation spectroscopy indicated that the release of SMP fractions in the early stage (0-150 min) changed in the following sequences: PN-like fraction > fulvic acid-like fraction.
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