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Related Concept Videos

Locus of Control01:26

Locus of Control

236
Locus of control describes how individuals perceive the causes of events in their lives, influencing motivation and well-being. Introduced by Julian Rotter in 1954, it is categorized into internal and external locus of control.Internal Locus of ControlIndividuals with an internal locus of control believe their actions determine outcomes, fostering responsibility, self-efficacy, and motivation. For example, an employee may attribute career success to hard work. Research links this mindset to...
236
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Root-Locus Method01:19

Root-Locus Method

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A cruise control system in a car is designed to maintain a specified speed automatically by adjusting the gas pedal. The system continuously measures the vehicle's speed and makes fine adjustments to the pedal to achieve this goal. The root locus method is particularly useful for understanding how the cruise control system's behavior changes under varying conditions, such as when the car goes uphill, downhill, or faces strong wind resistance.
This system can be represented by a block...
520
Construction of Root Locus01:15

Construction of Root Locus

425
The construction of a root locus involves several key steps to analyze and visualize the behavior of a system's poles with varying gain. The number of branches in the root locus equals the number of closed-loop poles and is symmetrical about the real axis.
For positive gain values, the root locus exists on the real axis to the left of an odd number of finite open-loop poles or zeros. The root locus starts at the open-loop poles and traces the paths of the closed-loop poles as the gain...
425
Properties of the Root Locus01:05

Properties of the Root Locus

308
The root locus method is an invaluable tool for analyzing higher-order systems without needing to factor the denominator of the transfer function. A pole of the system is identified when the characteristic polynomial in the transfer function's denominator equals zero.
To determine if a point lies on the root locus, the criterion involves the sum of angles contributed by all poles and zeros to that point. Specifically, this sum must be an odd multiple of 180 degrees. The gain at any point on...
308
Rotter's Locus of Control01:14

Rotter's Locus of Control

984
Julian Rotter introduced the concept of locus of control, a cognitive factor that significantly influences personality development and learning. Locus of control refers to an individual's beliefs about the extent of control they have over events in their lives. According to Rotter, this belief system can be categorized into two types: internal and external locus of control.
Individuals with an internal locus of control believe that their personal efforts and decisions directly affect their...
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Related Experiment Video

Updated: Feb 13, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

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Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing.

Elsa Leitão1, Jasmin Beygo1, Michael Zeschnigk1

  • 1Institute of Human Genetics, University of Duisburg-Essen, University Hospital Essen, Essen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2018
PubMed
Summary
This summary is machine-generated.

This study details a method for analyzing DNA methylation using targeted deep bisulfite sequencing. The approach enables precise determination and comparison of methylation levels in specific genomic regions and alleles.

Keywords:
Amplicon bisulfite sequencingAmplikyzer softwareDNA methylationMiSeq system

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DNA Methylation: Bisulphite Modification and Analysis

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

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Last Updated: Feb 13, 2026

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DNA Methylation: Bisulphite Modification and Analysis
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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

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Area of Science:

  • Epigenetics and Genomics
  • Molecular Biology

Background:

  • DNA methylation, the addition of a methyl group to cytosine at C5, is a key epigenetic mechanism regulating gene expression.
  • Understanding DNA methylation patterns is crucial for deciphering gene regulation and cellular function.

Purpose of the Study:

  • To present a comprehensive protocol for analyzing DNA methylation at single-molecule resolution using targeted deep bisulfite sequencing.
  • To provide a user-friendly method for analyzing complex multiplexed samples and comparing methylation data.

Main Methods:

  • Design of PCR primers for amplifying bisulfite-converted DNA.
  • Preparation and sequencing of targeted DNA libraries on the MiSeq platform.
  • Utilizing specialized software (amplikyzer2) for sequence read analysis.

Main Results:

  • Successful amplification and sequencing of bisulfite-converted DNA from targeted genomic regions.
  • Accurate determination of mean methylation values for CpG dinucleotides within regions and across samples.
  • Facilitated comparison of methylation status between different samples and alleles.

Conclusions:

  • Targeted deep bisulfite sequencing offers a robust method for single-molecule resolution DNA methylation analysis.
  • The described protocol and software tools simplify the analysis of complex epigenetic data, aiding in gene expression studies.