Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cellular Differentiation00:57

Cellular Differentiation

5.6K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.6K
iPS Cell Differentiation01:22

iPS Cell Differentiation

3.2K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.2K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

16.9K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
16.9K
What is Behavior?00:54

What is Behavior?

10.3K
Behaviors are actions that an organism engages in—they can be related to finding food, reproducing, defending against threats, and many other possible actions. Behaviors include activities related to the environment around the animal—such as migration—as well as social interactions within a species or population. Many behaviors involve motor output—that is, muscle movements—while others involve less visible actions, such as learning.
10.3K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

4.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
4.1K
Cellular Respiration01:18

Cellular Respiration

2.1K
Cellular respiration is a crucial metabolic process through which cells obtain energy from organic substances, mainly glucose, to produce adenosine triphosphate (ATP). This process includes the oxidation of substrates and the transfer of electrons to a separate electron acceptor, facilitating ATP synthesis through a sequence of biochemical reactions.Glycolysis: The Initial StepGlycolysis is the first stage of cellular respiration, occurring in the cytoplasm of both prokaryotic and eukaryotic...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic Atlas of Human Trabecular Meshwork Uncovers the Cellular Landscape and Provides Insights into Glaucoma Pathophysiology.

Research square·2026
Same author

Development and Validation of a Streamlined Workflow for Proteomic Analysis of Proteins and Post-translational Modifications from Dried Blood.

Journal of proteome research·2026
Same author

Composition Matters: Collagen vs Polyacrylamide Modulates Distinct Trabecular Meshwork Cell Traction.

ACS biomaterials science & engineering·2026
Same author

A prevalence study of Autism Spectrum Disorder in Russia.

Frontiers in psychiatry·2026
Same author

Segmental Mechanobiology of Normal and Glaucomatous Human Trabecular Meshwork Cells.

ACS measurement science au·2026
Same author

Macrophage Migration Inhibitory Factor (MIF)-CD74 Signaling Pathway Mediates Trabecular Meshwork Dysfunction in Glaucoma.

bioRxiv : the preprint server for biology·2026
Same journal

Corrigendum to "Senescent endothelial cells' response to the degradation of bioresorbable scaffold induces intimal dysfunction accelerating in-stent restenosis" [Acta Biomaterialia 166 (2023) 266-277].

Acta biomaterialia·2026
Same journal

Colorectum and embedded networks of nerve fibers present auxetic responses during uniaxial circumferential extension.

Acta biomaterialia·2026
Same journal

Music-inspired acoustic-piezoelectric stimulation accelerates extracellular vesicle production and programs therapeutic function.

Acta biomaterialia·2026
Same journal

Mutant superoxide dismutase 1-catalyzed hydrogen therapy for amyotrophic lateral sclerosis achieved by intercepting oxidative stress-neuroinflammation crosstalk.

Acta biomaterialia·2026
Same journal

Injectable pH-responsive gelatin methacryloyl hydrogel for cuproptosis-synergized sunitinib therapy and immune reprogramming in clear cell renal cell carcinoma.

Acta biomaterialia·2026
Same journal

Corrigendum to "Injectable hydrogel-assisted local lipopolysaccharide delivery improves immune checkpoint blockade therapy" [Acta Biomaterialia 2025, 194, 153-168].

Acta biomaterialia·2026
See all related articles

Related Experiment Video

Updated: Feb 13, 2026

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

10.5K

Glaucomatous cell derived matrices differentially modulate non-glaucomatous trabecular meshwork cellular behavior.

Vijay Krishna Raghunathan1, Julia Benoit2, Ramesh Kasetti3

  • 1Department of Basic Sciences, College of Optometry, University of Houston, Houston, TX 77204, United States; The Ocular Surface Institute, College of Optometry, University of Houston, Houston, TX 77204, United States.

Acta Biomaterialia
|March 11, 2018
PubMed
Summary
This summary is machine-generated.

Glaucoma involves stiffening of the trabecular meshwork (TM) extracellular matrix (ECM). Diseased TM ECM stiffens healthy cells, altering their function and mimicking glaucoma, highlighting ECM

Keywords:
Atomic force microscopyBiomechanicsCell derived matricesExtracellular matrixGlaucomaMechanotransductionTrabecular meshwork

More Related Videos

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.9K
Preparation of Complaint Matrices for Quantifying Cellular Contraction
11:38

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Published on: December 14, 2010

18.3K

Related Experiment Videos

Last Updated: Feb 13, 2026

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

10.5K
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.9K
Preparation of Complaint Matrices for Quantifying Cellular Contraction
11:38

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Published on: December 14, 2010

18.3K

Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Cell Biology

Background:

  • Ocular hypertension is a key risk factor for glaucoma.
  • Glaucoma is associated with trabecular meshwork (TM) stiffening, impacting aqueous humor outflow.
  • Mechanisms of TM stiffening and pathological extracellular matrix (ECM) effects on cell function are poorly understood.

Purpose of the Study:

  • To investigate the role of pathological ECM in glaucoma progression.
  • To characterize cell-derived matrices (CDMs) from glaucomatous and non-glaucomatous TM cells.
  • To determine the impact of glaucomatous ECM on normal TM cell behavior and phenotype.

Main Methods:

  • Utilized an anterior segment perfusion model to compare glaucomatous TM (GTM) and non-glaucomatous TM (NTM) tissue stiffness and flow.
  • Generated and characterized CDMs from GTM and NTM cells, focusing on fibronectin mechanics, composition, and organization.
  • Assessed the effects of GTM-derived ECM on NTM cell stiffening, gene/protein expression, endoplasmic reticular stress, and reorganization capacity.

Main Results:

  • GTM tissue exhibits stiffer, low-flow regions and fewer high-flow, less stiff regions compared to NTM.
  • GTM tissue has a reduced overall cell count compared to NTM.
  • GTM-derived ECM induced stiffening and altered gene/protein expression in normal NTM cells, mimicking a glaucomatous phenotype.
  • GTM-derived ECM caused endoplasmic reticular stress and impaired reorganization in NTM cells, with exacerbation by steroid-treated glaucoma models.

Conclusions:

  • Cell-derived matrices (CDMs) are a novel tool for studying cell-ECM interactions in glaucoma.
  • Pathological ECM in glaucoma contributes to disease progression by altering TM cell behavior and function.
  • Understanding these bidirectional interactions is crucial for developing glaucoma therapies.