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When one or more data points appear far from the rest of the data, there is a need to determine whether they are outliers and whether they should be eliminated from the data set to ensure an accurate representation of the measured value. In many cases, outliers arise from gross errors (or human errors) and do not accurately reflect the underlying phenomenon. In some cases, however, these apparent outliers reflect true phenomenological differences. In these cases, we can use statistical methods...
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Detection of Migrasomes.

Yang Chen1, Ying Li1, Liang Ma1

  • 1State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2018
PubMed
Summary

Migrasomes are newly discovered organelles crucial for cell communication and releasing cellular contents during migration. This study details methods for visualizing these unique structures using microscopy techniques.

Keywords:
Cell–cell communicationFibronectinFluorescence microscopyMigracytosisMigrasomeMigrationTransmission electron microscopy

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Area of Science:

  • Cell Biology
  • Organelle Biology
  • Cellular Communication

Background:

  • Migrasomes are recently identified, migration-dependent, membrane-bound organelles.
  • They play a role in releasing intracellular components and mediating cell-cell interactions.
  • Migrasomes possess distinct morphological characteristics differentiating them from other cellular structures.

Purpose of the Study:

  • To describe established methods for visualizing migrasomes.
  • To provide protocols for observing migrasomes using fluorescence and electron microscopy.

Main Methods:

  • Fluorescence microscopy techniques for migrasome visualization.
  • Electron microscopy protocols for detailed structural analysis of migrasomes.

Main Results:

  • Successful visualization of migrasomes using the described fluorescence microscopy methods.
  • Detailed morphological characterization of migrasomes via electron microscopy, highlighting their unique features.

Conclusions:

  • Established methods enable effective visualization and characterization of migrasomes.
  • Understanding migrasome morphology and visualization is key to studying their function in cell migration and communication.