Related Experiment Video

Updated: Feb 13, 2026

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy
07:13

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy

Published on: February 25, 2021

4.5K

Detection of Migrasomes

Yang Chen1, Ying Li1, Liang Ma1

  • 1State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2018
PubMed

Insights

Migrasomes are newly discovered organelles crucial for cell communication and releasing cellular contents during migration. This study details methods for visualizing these unique structures using microscopy techniques.

Area of Science:

  • Cell Biology
  • Organelle Biology
  • Cellular Communication

Background:

  • Migrasomes are recently identified, migration-dependent, membrane-bound organelles.
  • They play a role in releasing intracellular components and mediating cell-cell interactions.
  • Migrasomes possess distinct morphological characteristics differentiating them from other cellular structures.

Purpose of the Study:

  • To describe established methods for visualizing migrasomes.
  • To provide protocols for observing migrasomes using fluorescence and electron microscopy.

Main Methods:

  • Fluorescence microscopy techniques for migrasome visualization.
  • Electron microscopy protocols for detailed structural analysis of migrasomes.

Main Results:

  • Successful visualization of migrasomes using the described fluorescence microscopy methods.
  • Detailed morphological characterization of migrasomes via electron microscopy, highlighting their unique features.

Conclusions:

  • Established methods enable effective visualization and characterization of migrasomes.
  • Understanding migrasome morphology and visualization is key to studying their function in cell migration and communication.
Keywords:
Cell–cell communicationFibronectinFluorescence microscopyMigracytosisMigrasomeMigrationTransmission electron microscopy

More Related Videos

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce09:10

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Published on: October 18, 2010

13.5K
Detection of Protein Ubiquitination09:00

Detection of Protein Ubiquitination

Published on: August 19, 2009

43.7K

Related Concept Videos

Detection of Gross Error: The Q Test01:00

Detection of Gross Error: The Q Test

When one or more data points appear far from the rest of the data, there is a need to determine whether they are outliers and whether they should be eliminated from the data set to ensure an accurate representation of the measured value. In many cases, outliers arise from gross errors (or human errors) and do not accurately reflect the underlying phenomenon. In some cases, however, these apparent outliers reflect true phenomenological differences. In these cases, we can use statistical methods...
7.1K
Detection of Black Holes01:10

Detection of Black Holes

Although black holes were theoretically postulated in the 1920s, they remained outside the domain of observational astronomy until the 1970s.
Their closest cousins are neutron stars, which are composed almost entirely of neutrons packed against each other, making them extremely dense. A neutron star has the same mass as the Sun but its diameter is only a few kilometers. Therefore, the escape velocity from their surface is close to the speed of light.
Not until the 1960s, when the first neutron...
2.6K
Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
8.4K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
669
Types of Errors: Detection and Minimization01:12

Types of Errors: Detection and Minimization

Error is the deviation of the obtained result from the true, expected value or the estimated central value. Errors are expressed in absolute or relative terms.
Absolute error in a measurement is the numerical difference from the true or central value. Relative error is the ratio between absolute error and the true or central value, expressed as a percentage.
Errors can be classified by source, magnitude, and sign. There are three types of errors: systematic, random, and gross.
Systematic or...
11.5K
Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
6.0K