Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Products of the Citric Acid Cycle00:53

Products of the Citric Acid Cycle

103.7K
The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.
103.7K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.4K
Overview
80.4K
Weak Acid Solutions04:02

Weak Acid Solutions

43.4K
Few compounds act as strong acids. A far greater number of compounds behave as weak acids and only partially react with water, leaving a large majority of dissolved molecules in their original form and generating a relatively small amount of hydronium ions. Weak acids are commonly encountered in nature, being the substances partly responsible for the tangy taste of citrus fruits, the stinging sensation of insect bites, and the unpleasant smells associated with body odor. A familiar example of a...
43.4K
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Ions as Acids and Bases02:54

Ions as Acids and Bases

26.7K
Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
26.7K
Lewis Acids and Bases02:33

Lewis Acids and Bases

48.5K
In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
48.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Hemodynamic effects of synchronous and asynchronous independent lung ventilation with different levels of positive end-expiratory pressure and tidal volumes on unilateral lung injury in dogs].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2010
Same author

[Study on the immuno-effects and influencing factors of Chinese hamster ovary (CHO) cell hepatitis B vaccine among adults, under different dosages].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2010
Same author

Assemblies of fluorine containing bent-shaped liquid crystal molecules studied by using scanning tunneling microscopy.

Journal of nanoscience and nanotechnology·2010
Same author

Carbon nanotubes induce secondary structure changes of bovine albumin in aqueous phase.

Journal of nanoscience and nanotechnology·2010
Same author

[Immunogenicity and protective efficacy of pertactin recombinants against Bordetella bronchiseptica challenge].

Wei sheng wu xue bao = Acta microbiologica Sinica·2010
Same author

[Analysis of the electrocardiographic findings in 288 patients with acute pulmonary thromboembolism].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2010

Related Experiment Video

Updated: Feb 13, 2026

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
08:33

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Published on: April 11, 2021

4.9K

Enhancing oleanolic acid production in engineered Saccharomyces cerevisiae.

Yujia Zhao1, Jingjing Fan1, Chen Wang1

  • 1Institute for Synthetic Biosystem, Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.

Bioresource Technology
|March 13, 2018
PubMed
Summary

This study engineered Saccharomyces cerevisiae for enhanced oleanolic acid biosynthesis, achieving a 7.6-fold increase in production through pathway optimization and gene manipulation. The engineered yeast achieved a final production of 606.9 mg/L.

Keywords:
Galactose metabolic networkOleanolic acidOxidation-reduction system

More Related Videos

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

10.5K
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
09:06

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Published on: November 20, 2021

5.1K

Related Experiment Videos

Last Updated: Feb 13, 2026

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
08:33

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Published on: April 11, 2021

4.9K
Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

10.5K
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
09:06

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Published on: November 20, 2021

5.1K

Area of Science:

  • Biotechnology
  • Metabolic Engineering
  • Synthetic Biology

Background:

  • Oleanolic acid, a plant-derived triterpenoid, possesses diverse biological activities.
  • Microbial biosynthesis offers a sustainable route for oleanolic acid production, but yields are currently too low for industrial application.

Purpose of the Study:

  • To engineer Saccharomyces cerevisiae for significantly improved oleanolic acid production.
  • To overcome limitations in microbial oleanolic acid biosynthesis for industrial viability.

Main Methods:

  • Engineered a novel oleanolic acid biosynthesis pathway in yeast by enhancing enzyme complex efficiency.
  • Reconstructed the cellular galactose regulatory network by knocking out GAL80 and GAL1 to boost heterologous gene transcription.
  • Overexpressed key enzymes: 3-hydroxy-3-methylglutaryl-CoA reductase, squalene synthase, and 2,3-oxidosqualene synthase.
  • Optimized fermentation conditions to maximize oleanolic acid yield.

Main Results:

  • Achieved oleanolic acid production of 186.1 ± 12.4 mg/L in flask shake cultures.
  • Further increased production to 606.9 ± 9.1 mg/L with a yield of 16.0 ± 0.8 mg/g DCW after fermentation optimization.
  • Demonstrated a 7.6-fold increase compared to the previously reported maximum production levels.

Conclusions:

  • The engineered yeast strain and optimized fermentation process significantly enhance microbial oleanolic acid production.
  • This study presents a viable strategy for the industrial-scale biosynthesis of oleanolic acid.
  • The developed metabolic engineering approach provides a foundation for producing other valuable plant-derived compounds in microbes.