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Updated: Feb 13, 2026
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
A potential risk factor of essential hypertension in case-control study: Circular RNA hsa_circ_0037911
Xingjie Bao1, Shuying Zheng1, Shuqi Mao2
1Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medicine School of Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province, 315211, China.
Insights
Higher expression of hsa_circ_0037911 is linked to essential hypertension (EH). This circular RNA may play a role in EH development and can serve as a biomarker for early diagnosis.
Area of Science:
- Genetics and Molecular Biology
- Cardiovascular Disease Research
Background:
- Essential hypertension (EH) is a prevalent, multifactorial disease with unclear genetic regulatory mechanisms.
- Circular RNAs (circRNAs) are emerging as significant biomolecules for understanding EH pathogenesis.
- Research into the genetic basis of EH is in its early stages.
Purpose of the Study:
- To investigate the role of circRNAs in the molecular mechanisms of essential hypertension.
- To identify potential circRNA biomarkers for the early diagnosis of EH.
Main Methods:
- A case-control study involving 200 EH participants was conducted.
- circRNAs were selected and validated using quantitative real-time PCR (qRT-PCR).
- T-tests were employed to assess differential expression of circRNAs and microRNAs (miRNAs).
Main Results:
- The expression level of hsa_circ_0037911 was significantly higher in EH cases compared to healthy controls (p=0.005).
- hsa_circ_0037911 demonstrated effectiveness as a biomarker for EH (AUC=0.627, p=0.002).
- hsa_circ_0037911 levels correlated with gender, BMI, smoking, drinking, and serum creatinine (Scr) in EH patients.
Conclusions:
- Elevated hsa_circ_0037911 expression may be crucial in EH development, potentially by altering Scr concentrations.
- hsa_circ_0037911 shows promise as a stable biomarker for the early detection of essential hypertension.
Background:
Essential hypertension (EH) is a high prevalence with multifactorial diseases. Human studies on the impact of genes on this disease are just in the initial stage, the mechanism of gene regulation is still remains unclear. Circular RNAs (circRNAs) as a continuous cycle of covalent closure, RNA molecules added to the 3'-5' end covalently bound by the formation of incidental event. CircRNAs may be an important biomolecule in revealing the molecule regulate mechanisms of EH.
Methods:
The circRNAs were selected and validated with qRT-PCR followed. Our experiment was conducted with case-control studies among 200 EH participants. The t-test was used to evaluate the different expression of circRNAs and miRNAs, the significance of which was set as p < 0.05.
Results:
The hsa_circ_0037911 expression level in EH cases were significantly higher than healthy controls (p = 0.005). There was still important significance when adjusted by logistic regression (adjusted p = 0.026). We also found that hsa_circ_0037911 was an effective marker of EH (area under curve = 0.627; p = 0.002). The levels of hsa_circ_0037911 were significantly differences in gender, BMI, smoking and drinking among EH cases. There was a positive correlation between Serum creatinine (Scr) and hsa_circ_0037911.
Conclusions:
Our findings suggested that higher expression hsa_circ_0037911 may be key circRNAs for EH development by changing the concentration of Scr and could be a stable biomarker for early diagnosis of EH.
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