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Updated: Feb 13, 2026

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
Published on: September 20, 2022
Untargeted metabolomics reveals multiple metabolites influencing smoking-related DNA methylation
Yunfeng Huang1, Qin Hui1, Douglas I Walker2
1Department of Epidemiology, Emory University Rollins School of Public Health, Atlanta, GA 30322, USA.
Aim:
We conducted a joint metabolomic-epigenomic study to identify patterns of epigenetic associations with smoking-related metabolites.
Patients & Methods:
We performed an untargeted metabolome-wide association study of smoking and epigenome-wide association studies of smoking-related metabolites among 180 male twins. We examined the patterns of epigenetic association linked to smoking-related metabolites using hierarchical clustering.
Results:
Among 12 annotated smoking-related metabolites identified from a metabolome-wide association study, we observed significant hypomethylation associated with increased level of N-acetylpyrrolidine, cotinine, 5-hydroxycotinine and nicotine and hypermethylation associated with increased level of 8-oxoguanine. Hierarchical clustering revealed common and unique epigenetic-metabolic associations related to smoking.
Conclusion:
Our study suggested that a joint metabolome-epigenome approach can reveal additional details in molecular responses to the environmental exposure to understand disease risk.
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