Related Experiment Video

Updated: Feb 13, 2026

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
08:04

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Published on: March 18, 2014

13.3K

ING5 is a Potential Target for Osteosarcoma Therapy

Xing Zhang1, Zhong-Hua Xu1, Hua Xie1

  • 11 Department of Orthopedics, Jintan Hospital Affiliated to Jiangsu University, Jintan, Jiangsu, People's Republic of China.

Technology in Cancer Research & Treatment
|March 13, 2018
PubMed

Insights

Inhibitor of growth family of protein 5 (IGF5) is downregulated in osteosarcoma. Overexpressing IGF5 induces apoptosis and inhibits proliferation by activating the Smad pathway, suggesting its potential as an osteosarcoma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a common primary malignant bone tumor.
  • The inhibitor of growth family of protein 5 (IGF5) acts as a tumor suppressor in various cancers.
  • IGF5 downregulation is observed in osteosarcoma tissues.

Purpose of the Study:

  • To investigate the antitumor roles of IGF5 in osteosarcoma.
  • To determine the mechanism by which IGF5 affects osteosarcoma cell behavior.
  • To explore the potential of IGF5 as a therapeutic target for osteosarcoma.

Main Methods:

  • Western blot and real-time polymerase chain reaction to confirm IGF5 downregulation.
  • Transfection of osteosarcoma cell lines (HOS, MG63) with IGF5 plasmid.
  • Analysis of apoptosis, cell proliferation, and Smad pathway activation (p-Smad2, p-Smad3, Smad4).

Main Results:

  • IGF5 and its mRNA levels were confirmed to be downregulated in osteosarcoma tissues.
  • Overexpression of IGF5 in osteosarcoma cells induced apoptosis and inhibited cell proliferation.
  • Activation of the Smad pathway (increased p-Smad2, p-Smad3, and Smad4) was observed in IGF5-expressing cells.

Conclusions:

  • Overexpression of IGF5 induces apoptosis in osteosarcoma cells via Smad pathway activation.
  • IGF5 demonstrates significant antitumor effects in osteosarcoma.
  • IGF5 presents a promising therapeutic target for osteosarcoma treatment.
Keywords:
ING5apoptosisosteosarcomasurvival ratethe Smad pathway

More Related Videos

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.5K
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma09:25

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Published on: October 14, 2016

19.7K

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
9.0K
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.7K
Potential Energy00:52

Potential Energy

The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
42.9K
Standard Electrode Potentials03:02

Standard Electrode Potentials

On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
50.5K
Group Therapy01:26

Group Therapy

Group therapy is a sociocultural approach to psychological treatment, where individuals with shared psychological challenges come together under the guidance of a mental health professional. This therapeutic modality offers unique opportunities for individuals to connect, share, and grow within the context of a supportive group. By fostering mutual understanding and collaboration, group therapy can address a range of psychological concerns effectively, often complementing or surpassing the...
469
Cell Potential and Free Energy02:58

Cell Potential and Free Energy

Thermodynamics of a Redox Reaction
Thermodynamics is the branch of physics dealing with the relationship between heat and other forms of energy. In an electrochemical cell, chemical energy is converted into electrical energy.
Thus, a link can be predicted between cell potential, free energy change, and the equilibrium constant for the reaction. Cell potential can also be measured as the oxidant or the reducing strength, and similar acid-base strength measures are reflected in equilibrium...
46.8K