Alignment of single-cell trajectories to compare cellular expression dynamics
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Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis
Published on: May 20, 2012
Ayelet Alpert1, Lindsay S Moore1, Tania Dubovik1
1Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel.
Abstract:
Single-cell RNA sequencing and high-dimensional cytometry can be used to generate detailed trajectories of dynamic biological processes such as differentiation or development. Here we present cellAlign, a quantitative framework for comparing expression dynamics within and between single-cell trajectories. By applying cellAlign to mouse and human embryonic developmental trajectories, we systematically delineate differences in the temporal regulation of gene expression programs that would otherwise be masked.
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