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Can the quorum sensing inhibitor resveratrol function as an aminoglycoside antibiotic accelerant against Pseudomonas

Jin-Wei Zhou1, Tong-Tong Chen1, Xiao-Juan Tan2

  • 1State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry Education, Hainan University, Haikou 570228, China; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

International Journal of Antimicrobial Agents
|March 14, 2018
PubMed

Insights

Resveratrol, a quorum sensing inhibitor, enhances aminoglycoside antibiotic effectiveness against Pseudomonas aeruginosa biofilms. This combination therapy shows promise for treating persistent infections in cystic fibrosis patients.

Area of Science:

  • Microbiology
  • Pharmacology
  • Biotechnology

Background:

  • Pseudomonas aeruginosa infections are challenging in cystic fibrosis due to biofilms and antibiotic resistance.
  • Quorum sensing (QS) plays a critical role in P. aeruginosa virulence and biofilm formation.

Purpose of the Study:

  • To investigate the synergistic effects of resveratrol, a QS inhibitor, and aminoglycoside antibiotics against P. aeruginosa biofilms.
  • To evaluate resveratrol's potential to restore or enhance antibiotic efficacy in treating P. aeruginosa infections.

Main Methods:

  • Crystal violet staining assay to assess biofilm susceptibility.
  • Scanning electron and fluorescence microscopy for biofilm structural analysis.
  • Quantitative reverse transcription PCR (qRT-PCR) to analyze gene expression related to QS.

Main Results:

  • Resveratrol treatment increased P. aeruginosa biofilm susceptibility to aminoglycoside antibiotics.
  • Combined resveratrol and aminoglycoside therapy disrupted biofilm architecture.
  • Resveratrol inhibited the expression of lasI and rhlI genes involved in P. aeruginosa QS.

Conclusions:

  • Resveratrol significantly enhances the efficacy of aminoglycoside antibiotics against P. aeruginosa biofilms.
  • Resveratrol acts as a potential therapeutic accelerant, improving treatment outcomes for P. aeruginosa infections.
Keywords:
Aminoglycoside antibioticsAntibiotic accelerantBiofilmPseudomonas aeruginosaQuorum sensing

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