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Related Experiment Videos

Histological changes underlying bupivacaine's effect on extra ocular muscle.

Luisa Moreira Hopker1, Juliana de Carvalho Neves2, Daiane Jaqueline Nascimento3

  • 1Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil; Evangelical Hospital of Curitiba, Curitiba, Brazil.

Experimental Eye Research
|March 14, 2018
PubMed
Summary

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Bupivacaine injection in rabbit extraocular muscles initially shrinks myofibers, then normalizes them, with a temporary rise in slow myosin fibers. Untreated muscles adapt with increased size, aiding strabismus correction understanding.

Area of Science:

  • Ophthalmology
  • Muscle Physiology
  • Surgical Research

Background:

  • Bupivacaine (BUP) injections are used in strabismus correction.
  • Understanding BUP's effects on extraocular muscle (EOM) structure is crucial for clinical application.
  • Changes in myofiber cross-sectional area (CSA) and myosin isoform distribution are key indicators of muscle adaptation.

Purpose of the Study:

  • To investigate the impact of BUP injection on rabbit EOM myofiber CSA and subtype distribution.
  • To correlate these structural changes with myosin isoform expression over time.
  • To provide insights into the clinical efficacy of BUP for strabismus treatment.

Main Methods:

  • Rabbits received BUP injections in the superior rectus (SR) muscle; control groups were used.
Keywords:
BUPBupivacaineCSACross-sectional areaExtraocular muscleIRODIROSInferior rectus of left eyeInferior rectus of right eyeMuscle physiologyPDPrism dioptersRegenerationSRODSROSSlow myosinStrabismusSuperior rectus of left eyeSuperior rectus of right eye

Related Experiment Videos

  • Histological analysis (Hematoxylin and eosin) and immunohistochemistry for myosin isoforms (slow, fast, embryonic) were performed.
  • Myofiber CSA was measured using ImageJ software at 7, 28, 60, and 92 days post-injection.

    • Main Results:

    • BUP injection initially decreased SR myofiber CSA, followed by a trend of increase and normalization by day 92.
    • A transient increase in slow myosin-positive fibers was observed at day 60.
    • Untreated EOM showed increased CSA, indicating adaptive changes.

    Conclusions:

    • BUP causes initial myofiber atrophy followed by recovery and normalization in EOM.
    • A temporary shift towards slow myosin expression occurs post-BUP injection.
    • EOM exhibits adaptive plasticity, which is relevant for understanding BUP's role in strabismus surgery.