Related Experiment Video
Updated: Feb 13, 2026

Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
Published on: January 16, 2019
Maximum-likelihood determination of anomalous substructures
Randy J Read1, Airlie J McCoy1
1Department of Haematology, University of Cambridge, Hills Road, Cambridge CB2 0XY, England.
Abstract:
A fast Fourier transform (FFT) method is described for determining the substructure of anomalously scattering atoms in macromolecular crystals that allows successful structure determination by X-ray single-wavelength anomalous diffraction (SAD). This method is based on the maximum-likelihood SAD phasing function, which accounts for measurement errors and for correlations between the observed and calculated Bijvoet mates. Proof of principle is shown that this method can improve determination of the anomalously scattering substructure in challenging cases where the anomalous scattering from the substructure is weak but the substructure also constitutes a significant fraction of the real scattering. The method is deterministic and can be fast compared with existing multi-trial dual-space methods for SAD substructure determination.
More Related Videos
05:22Dissociation of the Confounding Influences of Expectancy and Integrative Difficulty Residing in Anomalous Sentences in Event-related Potential Studies
Published on: May 9, 2019
14:28Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
Published on: July 15, 2020
Related Concept Videos
Determination of Michaelis Constant and Maximum Elimination Rate
These parameters can be estimated by analyzing plasma concentration data post-drug administration. A notable example of this application is phenytoin, a drug with capacity-limited kinetics. It's recommended that phenytoin should be administered at two...
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
Maximum Deflection
The maximum deflection occurs at a specific point, known as point O, where the tangent to the deflection curve is horizontal. To find point O, the slope of the tangent at any...
Maximum Power Transfer
By substituting the entire circuit with...
Maximum Size of Aggregate
The Maximum Power Transfer Theorem
The load connected draws the current, and the circuit delivers the power to the load. The alternating current flowing through the load is determined using the rectangular form of voltages, currents, network impedance, and load impedance. The average power delivered to the load is obtained from the product of the square of current and load resistance.