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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

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[Antibiotic Resistance of Enterococci Isolated from Healthy Humans and Patients with Various Pathologies.]

M V Sycheva, O L Kartashova, N E Shchepitova

    Antibiotiki I Khimioterapiia = Antibiotics and Chemoterapy [Sic]
    |March 14, 2018
    PubMed

    Insights

    Enterococci exhibit high resistance to common antibiotics. However, streptomycin and gentamicin remain effective against clinical and intestinal isolates, despite the presence of resistance genes.

    Area of Science:

    • Microbiology
    • Antimicrobial Resistance
    • Genetics

    Background:

    • Enterococci are significant human pathogens, frequently associated with hospital-acquired infections.
    • Increasing antimicrobial resistance in Enterococcus species poses a substantial threat to public health.
    • Limited treatment options are available for infections caused by multidrug-resistant enterococci.

    Purpose of the Study:

    • To evaluate the antimicrobial susceptibility of clinical and intestinal Enterococcus isolates.
    • To identify the genetic basis of antibiotic resistance in these isolates.
    • To compare phenotypic resistance with genotypic resistance patterns.

    Main Methods:

    • Bacteriological testing of Enterococcus isolates against various antibiotics.
    • Polymerase Chain Reaction (PCR) to detect specific antibiotic resistance genes.
    • Comparative analysis of phenotypic and genotypic resistance data.

    Main Results:

    • High resistance observed against tetracycline, ciprofloxacin, and ampicillin.
    • Streptomycin, gentamicin, and vancomycin demonstrated the highest activity against clinical isolates.
    • Streptomycin and gentamicin were most active against intestinal isolates.
    • PCR confirmed the presence of genes conferring resistance to aminoglycosides, glycopeptides, and tetracycline.
    • Identified isolates with resistance genes but without significant clinical resistance phenotypes.

    Conclusions:

    • Streptomycin and gentamicin are promising therapeutic options for enterococcal infections.
    • The presence of resistance genes without corresponding clinical resistance highlights a critical stage in resistance development.
    • Continuous monitoring of antimicrobial susceptibility and resistance mechanisms is crucial for effective treatment strategies.

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