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Related Concept Videos

Data Collection II01:29

Data Collection II

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The nursing history captures and records the patient's health status, so that a care plan evolves to meet the patient's individual needs. The nursing health history is a part of the initial assessment. A comprehensive history covers all health dimensions and plays a significant role in the assessment process. A comprehensive history includes the patient's biographical information, reasons for seeking health care, expectations, present and past health history, medications, and...
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Data Collection I01:30

Data Collection I

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Data collection gathers information needed to make accurate judgments about a patient's present condition. During a health history interview, subjective data is collected from the patient, their caregivers, or family members, and objective data is collected through observations and physical assessment. Patients are the primary source of subjective data. Thus information gathered from patients through interviews, observations, and physical examination is primary data. Secondary sources of...
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Data Collection by Experiments01:13

Data Collection by Experiments

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Data collection is a systematic method of obtaining, observing, measuring, and analyzing accurate information. An experimental study is a standard method of data collection that involves the manipulation of the samples by applying some form of treatment prior to data collection. It refers to manipulating one variable to determine its changes on another variable. The sample subjected to treatment is known as “experimental units.”
An example of the experimental method is a public...
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Data Collection by Survey01:07

Data Collection by Survey

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The systematic method of obtaining and analyzing accurate information of a population is called data collection. A survey is a standard method of data collection that involves collecting information from a target human population about their experience, opinion, or knowledge of a product, service, or process. The responses are recorded and interpreted. The most common survey examples are written questionnaires, face-to-face or telephonic conversations, focus groups, and electronic (e-mail or...
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Data Collection by Observations01:08

Data Collection by Observations

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Data collection refers to a systematic way of obtaining, observing, measuring, and analyzing accurate information. Observational studies are one of the most widely used methods of data collection. It involves collecting data by observing the behavior and physical characteristics of a sample without making any modifications to the sample.
An astronomer viewing the motion and brightness of stars in the sky and recording the data is an example of observational data collection. A botanist recording...
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Data Collection III01:05

Data Collection III

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The physical assessment examines the patient for objective data that defines the patient's condition, and aids in formulating the nursing care plan. The purpose of physical assessment is a health status appraisal, which includes identifying health problems, and establishing a database for nursing intervention.
The principles to begin the physical assessment include conducting a comprehensive or problem-related history in a quiet, well-lit room, emphasizing privacy and comfort for the...
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Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
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Platforms for Single-Cell Collection and Analysis.

Lukas Valihrach1, Peter Androvic2,3, Mikael Kubista4,5

  • 1Laboratory of Gene Expression, Institute of Biotechnology CAS, Biocev, Vestec 252 50, Czech Republic. lukas.valihrach@ibt.cas.cz.

International Journal of Molecular Sciences
|March 15, 2018
PubMed
Summary
This summary is machine-generated.

High-quality single-cell isolation is crucial for accurate biological analysis. This review covers advanced methods, including microfluidics and droplet techniques, enhancing cell analysis and rare cell characterization.

Keywords:
analysiscollectionisolationsingle cell

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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Single-cell analysis is vital for understanding cell heterogeneity and rare cell populations.
  • Its applications are expanding across all biological fields, driving innovation in tools and techniques.
  • High-quality single-cell isolation is essential for maintaining native expression profiles.

Purpose of the Study:

  • To review current methods and recent developments in single-cell isolation techniques.
  • To discuss the advantages and applicability of various commercially available platforms.
  • To provide insights into future directions in single-cell isolation technology.

Main Methods:

  • Classical methods: micromanipulation, microdissection, fluorescence-activated cell sorting (FACS).
  • New approaches: microfluidic chips, droplets, micro-well plates.
  • Automated cell collection: capillaries, magnets, electric fields, punching probes.

Main Results:

  • Significant advancements have been made in single-cell isolation technologies.
  • New techniques offer improved sensitivity, throughput, and gentleness compared to traditional methods.
  • Various platforms provide diverse options for specific research needs.

Conclusions:

  • The field of single-cell isolation is rapidly evolving with innovative technologies.
  • These advancements are critical for the increasing demands of single-cell RNA sequencing and bioinformatic analysis.
  • Future developments promise further improvements in efficiency and applicability.