Related Experiment Video
Updated: Feb 13, 2026

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
Published on: November 28, 2016
Which of low-density lipoprotein cholesterol estimates can be used in children with type 1 diabetes?
Jolanta Bugajska1, Joanna Berska1, Katarzyna Mamica1
11Department of Clinical Biochemistry, Jagiellonian University Medical College, Krakow, Poland.
Background:
One of the strongest risk factors of cardiovascular disease is a high concentration of low-density lipoprotein cholesterol (LDL-C); thus, the accurate measurement of LDL-C concentration in children is important. The aim of this study was to compare the concentration of LDL-C measured by direct method and the LDL-C concentration estimated by different formulas with the modified β-quantification method in children with type 1 diabetes.
Methods:
Thirty-one serum samples received from diabetic children (15/18; M/F) and 26 serum samples taken from nondiabetic children (18/14; M/F) were used in the study. LDL-C concentrations were determined by direct enzymatic method (LDL-CD) and the modified β-quantification method (LDL-CmBQ). The concentration of LDL-C was also calculated by different formulas.
Results:
The results of LDL-CmBQ and LDL-CD correlated. However, the correlation coefficient obtain in the nondiabetic samples was much higher (r=0.927; p<0.001) than the correlation coefficient for LDL-CmBQ and LDL-CD concentration obtained in the diabetic samples (r=0.691; p<0.001). Additionally, the coefficients of correlation between the LDL-CmBQ concentration and the LDL-C concentrations calculated by different formulas were lower in diabetic (range: 0.514-0.693) than in nondiabetic samples (range: 0.834-0.937). Bland-Altman plots showed much higher confidence intervals for 95% limits of agreement for the differences between LDL-CmBQ and LDL-CD as well as for the differences between LDL-CmBQ and LDL-C estimated by different formulas for samples from the diabetic samples compared with nondiabetic samples.
Conclusions:
The limitations of methods measurement and of each of the formulas should be taken into account when a medical decision to lower LDL-C is made in the treatment of diabetic children.
Related Concept Videos
Diabetes Mellitus: Type 2 and Gestational
Diabetes Mellitus: Overview and Type I Subtype
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
Cholesterol: Significance and Regulation
Considering cholesterol and...
Types of Hormones
What are Estimates?
The estimate for the mean of a sample is denoted by ͞x, whereas the mean of the population is designated as μ. Further, parameters such...
Pathophysiology of Diabetes
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...

