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Updated: Feb 13, 2026

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
Published on: August 1, 2022
Using a kidney pump to perfuse a free filet flap for reconstruction after hemipelvectomy: A case report
Katharine M Hinchcliff1, Jessica Crockett1, Steven W Thorpe2
1Division of Plastic Surgery, University of California-Davis Medical Center, Sacramento, California.
Abstract:
Long ischemia times adversely affect free flap survival, and large muscle flaps are particularly vulnerable. Hypothermic machine perfusion (HMP) is a well-established method of organ preservation, and recent literature has detailed the use of HMP to extend free flap ischemia times, predominantly in the laboratory setting. One limitation in the study and adoption of free flap HMP has been the availability of standardized perfusion machinery, as thus far institutions have built their own devices. We present a case of a 75-year-old woman with dedifferentiated chondrosarcoma of her right proximal femur. She underwent a "spare parts" surgery using a filet flap from her lower leg. Due to an obligate long ischemia time between flap harvest and revascularization, a kidney transplant perfusion pump was used to flush and cool the flap. After completion of the disarticulation, free tissue transfer proceeded successfully. The patient had an uncomplicated post-operative course until developing a local recurrence at three months post-resection. We believe that the perfusion technology already created for solid organ transplant will have expanding indications in the future of free tissue transfer and limb replantation.
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