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Related Experiment Videos

Ca2+-activated ATPase in microsomes from human liver.

C Spamer, C Heilmann, W Gerok

    The Journal of Biological Chemistry
    |June 5, 1987
    PubMed
    Summary

    Human liver microsomes show ATP-dependent calcium uptake, indicating a Ca2+-ATPase that may function in the endoplasmic reticulum. This enzyme

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Physiology

    Background:

    • Human liver microsomes possess ATP-supported Ca2+ uptake.
    • Ca2+ uptake is linked to Ca2+-stimulated ATPase activity.

    Purpose of the Study:

    • To investigate the mechanism of Ca2+ uptake and Ca2+-ATPase activity in human liver microsomes.
    • To characterize the Ca2+-ATPase enzyme and its reaction mechanism.

    Main Methods:

    • ATP-supported Ca2+ uptake assays.
    • Ca2+-stimulated ATPase activity measurements.
    • Enzyme phosphorylation studies and proteolysis analysis.

    Main Results:

    • Ca2+ uptake and ATPase activity were characterized with half-maximal concentrations.
    • A Mr = 116,000 phosphoprotein intermediate of the Ca2+-ATPase was identified.
    • The reaction mechanism involves ADP-reactive and ADP-unreactive phosphoenzyme intermediates.

    Conclusions:

    • The human liver Ca2+-ATPase appears to be an E1P·E2P-type enzyme.
    • This enzyme is likely a Ca2+-transport ATPase operating in the endoplasmic reticulum of human liver cells.

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