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Isolation and Cultivation of Adult Rat Cardiomyocytes
Published on: October 19, 2017
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Bioengineering Cardiac Tissue Constructs With Adult Rat Cardiomyocytes
Ze-Wei Tao1,2, Mohamed Mohamed2, Jeffrey G Jacot1
1From the Department of Bioengineering, University of Colorado Denver/Anschutz Medical Campus, Aurora, Colorado.
ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|March 15, 2018
Summary
Bioengineered cardiac tissue constructs using adult rat cardiomyocytes show promise for treating heart defects. Indirectly plated, dedifferentiated cells yielded superior electromechanical properties compared to freshly isolated cells.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Cardiovascular Research
Background:
- Adult cardiomyocytes are crucial for cardiac tissue engineering.
- Existing methods face challenges in creating functional cardiac constructs.
- Developing robust bioengineered cardiac tissues is vital for treating heart conditions.
Purpose of the Study:
- To bioengineer functional cardiac tissue constructs using adult rat cardiomyocytes.
- To analyze and compare the electromechanical properties of constructs made with dedifferentiated versus fresh cardiomyocytes.
- To establish a reliable protocol for generating three-dimensional cardiac tissues.
Main Methods:
- Adult rat cardiomyocytes were isolated using collagenase type II and Langendorff perfusion.
- Cardiac tissue constructs were fabricated on a fibrin gel matrix.
- Two plating methods were used: indirect plating of dedifferentiated cells and direct plating of fresh cells.
Main Results:
- Both construct types contracted for up to 30 days, exhibiting detectable electrogram (ECG) signals and contractile forces.
- Indirectly plated, dedifferentiated cardiomyocyte constructs showed significantly higher ECG R wave amplitude (15.1 ± 5.2 µV) and twitch force (70-110 µN) at a faster rate (~390 bpm).
- Directly plated fresh cardiomyocyte constructs exhibited lower ECG R wave amplitude (6.3 ± 2.5 µV), twitch force (40-60 µN), and contraction rate (~230 bpm).
Conclusions:
- Successfully bioengineered three-dimensional cardiac tissue constructs using primary adult cardiomyocytes.
- Indirect plating of dedifferentiated cardiomyocytes significantly enhances the electromechanical function of cardiac tissue constructs.
- This study provides a foundation for developing advanced cardiac tissue engineering strategies for therapeutic applications.
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