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Three new sesquiterpenes from Ainsliaea glabra
Ya-Ping Chen1,2, Cui Tong1, Wen-Quan Lu1
1a Department of Pharmacy , Changzheng Hospital, Second Military Medical University , Shanghai , China.
Natural Product Research
|March 16, 2018
Summary
Ainsliaea glabra yielded three new sesquiterpenes. Compound 2 demonstrated significant anti-inflammatory effects by inhibiting nuclear factor kappa B (NF-κB) and reducing pro-inflammatory cytokines.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Immunology
Background:
- Ainsliaea glabra is a plant source of bioactive compounds.
- Sesquiterpenes are a class of natural products with diverse biological activities.
- Inflammation is a key process in many diseases, making anti-inflammatory agents a focus of research.
Purpose of the Study:
- To isolate and characterize new compounds from Ainsliaea glabra.
- To evaluate the anti-inflammatory potential of isolated compounds.
- To investigate the mechanism of action of active compounds.
Main Methods:
- Isolation and structural elucidation of compounds using 1D/2D NMR and HR-ESIMS.
- Inhibition assay of nuclear factor kappa B (NF-κB) using a luciferase reporter cell line stimulated by lipopolysaccharide (LPS).
- Measurement of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-10) in LPS-stimulated macrophages.
Main Results:
- Three new sesquiterpenes (1-3) and eight known compounds (4-11) were isolated.
- Compound 2 (ainsliaea acid A) showed significant inhibition of NF-κB activation.
- Compound 2 reduced the production of tumor necrosis factor-α, interleukin-1β, IL-6, and IL-10.
Conclusions:
- Ainsliaea glabra is a rich source of novel sesquiterpenes.
- Ainsliaea acid A possesses potent anti-inflammatory properties.
- Compound 2 warrants further investigation as a potential therapeutic agent for inflammatory conditions.
