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Updated: Feb 13, 2026

High-Throughput Protein Crystallization via Microdialysis
Published on: March 3, 2023
An Inexpensive, In-House-Made, Microdialysis Device for Measuring Drug-Protein Binding
Zackary M Herbst1, Sayaka Shibata2, Erkang Fan2
1Division of Allergy and Infectious Disease, Department of Medicine, University of Washington, Seattle, Washington 98195, United States.
Abstract:
An inexpensive, in-house made microdialysis device is described that is suitable for measuring the binding of small molecules including drug candidates to serum proteins or other macromolecules. The device is based on the standard equilibrium dialysis method to measure the fraction of low molecular weight compound bound to proteins. It is constructed from a standard polypropylene 96-well plate, dialysis tubing, and low viscosity epoxy resin. The device can be readily prepared for a small fraction of the cost of a commercial, multichamber microdialysis device. Drug-protein binding results are provided, which validates the device.
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