Related Experiment Video
Updated: Feb 13, 2026

Laser-induced Forward Transfer of Ag Nanopaste
Published on: March 31, 2016
Improving the Cell Viability and Isolating Precision of Laser-induced Forward Transfer Process by Maintaining a
Yu Deng1,2, Zhigang Huang1, Wenbing Wang1
1School of Electromechanical Engineering, Guangdong University of Technology, Guangzhou, China.
Background:
Aiming to improve the laser-induced forward transfer (LIFT) cell isolation process, a polydimethylsiloxane (PDMS) layer with micro-hole arrays was employed to improve the cell separation precision, and a microchip with heater was developed to maintain the working area at 100% humidity and 37°C with the purpose to preserve the viability of the isolated cells.
Methods:
A series of experiments were conducted to verify the contributions of the optimization to LIFT cell isolation process as well as to study the effect of laser pulse energy, laser spot size and the titanium thickness on cell isolation. With 40µm laser spot size and 40nm thick of titanium, laser energy threshold for 100% single cell isolating succeed ratio is 7µJ.
Results:
According to the staining images and proliferation ratios, the chip did help to improve the cell availability and the cells can recover from the juries at least a day earlier comparing to the samples processed without the chip.
Conclusion:
With a Lattice Boltzmann model, the cell isolation process is numerically studied and it turns out that the micro-hole makes the isolation process shift to a micro-syringe injection model leading to the lower laser energy threshold for cell separation and fewer injuries.
Related Concept Videos
Cyclic Processes And Isolated Systems
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
Overview of Connective Tissues Proper
The loose connective tissues have a meshwork of thin collagen and elastin fibers, which provide tensile strength for support and enough elasticity to move...
Uncertainty in Measurement: Accuracy and Precision
Layers of Connective Tissue Proper
Improving Translational Accuracy
Design Example: Maintaining Level of an Embankment

