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Updated: Feb 13, 2026

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
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Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Published on: May 8, 2018

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The CD11a and Endothelial Protein C Receptor Marker Combination Simplifies and Improves the Purification of Mouse

Alborz Karimzadeh1,2, Vanessa M Scarfone1, Erika Varady1,2

  • 1Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, California, USA.

Stem Cells Translational Medicine
|March 16, 2018
PubMed

Hematopoietic stem cells (HSCs) are the self-renewing multipotent progenitors to all blood cell types. Identification and isolation of HSCs for study has depended on the expression of combinations of surface markers on HSCs that reliably distinguish them from other cell types. However, the increasing number of markers required to isolate HSCs has made it tedious, expensive, and difficult for newcomers, suggesting the need for a simpler panel of HSC markers. We previously showed that phenotypic HSCs could be separated based on expression of CD11a and that only the CD11a negative fraction contained true HSCs. Here, we show that CD11a and another HSC marker, endothelial protein C receptor (EPCR), can be used to effectively identify and purify HSCs. We introduce a new two-color HSC sorting method that can highly enrich for HSCs with efficiencies comparable to the gold standard combination of CD150 and CD48. Our results demonstrate that adding CD11a and EPCR to the HSC biologist's toolkit improves the purity of and simplifies isolation of HSCs. Stem Cells Translational Medicine 2018;7:468-476.

Keywords:
Bone marrow transplantationCell surface markersHematopoietic stem cellslong-term repopulation

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