Related Experiment Video
Updated: Feb 13, 2026
06:03
Isolation and Characterization of Microvesicles from Peripheral Blood
Published on: January 6, 2017
23.8K
Aβ42 Protofibrils Interact with and Are Trafficked through Microglial-Derived Microvesicles.
Lisa K Gouwens1, Mudar S Ismail1, Victoria A Rogers1
1Department of Chemistry and Biochemistry , University of Missouri-St. Louis , St. Louis , Missouri 63121 , United States.
ACS Chemical Neuroscience
|March 16, 2018
Summary
Microvesicles (MVs) shed from microglia interact with amyloid-beta. These MVs carry internalized amyloid-beta, potentially influencing Alzheimer's disease neuroinflammation and neurotoxicity.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- Extracellular vesicles (EVs), including microvesicles (MVs) and exosomes, mediate cell-to-cell communication.
- MVs and exosomes are implicated in neurodegenerative diseases like Alzheimer's disease (AD).
- Increased MV numbers are observed in AD, with interactions with amyloid-beta (Aβ) peptides.
Purpose of the Study:
- To characterize MVs shed from microglial cells.
- To understand MV interactions with Aβ.
- To determine if internalized Aβ is incorporated into secreted MVs.
Main Methods:
- Characterization of MVs from BV-2 microglia using ATP stimulation.
- Confocal imaging, dynamic light scattering, and electron microscopy for MV analysis.
- Aβ ELISA and fluorescent membrane probes to study MV-Aβ interactions.
Main Results:
- MVs displayed diameters ranging from 150 to 600 nm.
- MVs showed strong interaction with Aβ protofibrils but not monomers.
- Microglia internalized Aβ protofibrils, and secreted MVs contained these protofibrils.
Conclusions:
- Microglia-derived MVs interact with Aβ protofibrils.
- Internalized Aβ can be released via MVs.
- MV-Aβ interactions may play a role in AD neuroinflammation and neurotoxicity.
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