Related Experiment Video

Updated: Feb 13, 2026

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
10:35

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

Published on: November 28, 2010

18.9K

Characterization of the Ovarian Tumor Peptidome

Tao Liu1, Karin D Rodland1, Richard D Smith1

  • 1Pacific Northwest National Laboratory, Richland, WA, United States.

Vitamins and Hormones
|March 17, 2018
PubMed

Insights

Researchers developed a new platform for analyzing tumor peptidomes, improving cancer research. This method enhances the identification and quantification of proteins, offering new insights into ovarian cancer.

Area of Science:

  • Biochemistry
  • Oncology
  • Proteomics

Background:

  • Aberrant protein degradation is linked to various diseases, including cancer.
  • Tumor peptidome analysis offers insights into cancer's proteolytic processing.
  • Previous peptidomics research in cancer is limited by identification/quantification challenges and sample biases.

Purpose of the Study:

  • To develop a robust analytical platform and informatics approach for comprehensive tissue peptidome analysis.
  • To enable high-throughput, comprehensive, and quantitative peptidomics analysis.
  • To assess the suitability of clinical ovarian tumor samples for peptidomics.

Main Methods:

  • Development of a novel analytical platform for peptidomics.
  • Implementation of a new informatics approach for data analysis.
  • Analysis of clinical ovarian tumor samples with strict pre-processing controls.

Main Results:

  • Demonstrated a high-throughput, comprehensive, and quantitative peptidomics pipeline.
  • Confirmed the suitability of ovarian tumor samples processed within 60 minutes of excision.
  • Established a foundation for detailed molecular and functional analyses of ovarian cancer peptidomics.

Conclusions:

  • The developed peptidomics pipeline overcomes previous limitations in cancer research.
  • This approach enables robust analysis of clinical tissue samples.
  • Further studies can now explore the molecular details and functional significance of peptidomic activities in ovarian cancer.
Keywords:
IdentificationInformed quantitationIschemiaMS-GF+Ovarian cancerPeptidomicsProteasesProtein degradationQuantificationTumor

More Related Videos

Characterization and Functional Prediction of Bacteria in Ovarian Tissues10:12

Characterization and Functional Prediction of Bacteria in Ovarian Tissues

Published on: October 23, 2021

3.2K
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells11:37

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

Published on: February 18, 2015

14.3K

Related Concept Videos

Ovarian Cycle01:27

Ovarian Cycle

The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
4.2K
Hormonal Control of the Ovarian Cycle01:30

Hormonal Control of the Ovarian Cycle

The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle.  At puberty, GnRH secretion increases in both frequency and...
7.0K
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
2.0K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.5K
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.9K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.0K