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Blood periovulatory progesterone quantification using different techniques in the dog.

Alessia Gloria1, Alberto Contri1, Augusto Carluccio1

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Summary
This summary is machine-generated.

Measuring blood progesterone in female dogs requires careful method selection. Radioimmunoassay (RIA), chemiluminescent immunoassay (CLIA), and enzyme-linked immunosorbent assay (ELISA) yield different results, impacting reproductive management decisions.

Keywords:
BitchChemiluminescence immunoassayEnzyme linked immunosorbed assayProgesteroneRadioimmunoAssay

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Area of Science:

  • Veterinary Medicine
  • Reproductive Biology
  • Clinical Pathology

Background:

  • Blood progesterone concentration is crucial for canine reproductive management, including ovulation timing and pregnancy monitoring.
  • Accurate progesterone measurement is essential for successful assisted reproductive technologies and diagnosing reproductive disorders in bitches.
  • Existing methods like radioimmunoassay (RIA), chemiluminescent immunoassay (CLIA), and enzyme-linked immunosorbent assay (ELISA) vary in their reported values.

Purpose of the Study:

  • To compare blood progesterone concentrations measured by RIA, CLIA, and ELISA during the periovulatory period in female dogs.
  • To evaluate the agreement and correlation between these three common progesterone assay methods.
  • To determine the influence of the chosen assay method on progesterone values during critical reproductive phases.

Main Methods:

  • Blood samples were collected from 23 female dogs during proestrus and every other day between cytologic estrus and cytologic diestrus.
  • Vaginal cytology was used to precisely stage the estrous cycle phases.
  • Progesterone concentrations were retrospectively analyzed using RIA, CLIA, and ELISA.

Main Results:

  • A significant increase in circulating progesterone was observed as the estrous cycle progressed.
  • Progesterone values differed significantly across the three methods: RIA yielded the lowest, CLIA intermediate, and ELISA the highest values.
  • CLIA and RIA showed high correlation (r=0.978) and strong agreement (CCC=0.966), while ELISA and RIA showed high correlation (r=0.955) but only moderate agreement (CCC=0.866).

Conclusions:

  • The method employed for measuring blood progesterone concentration significantly impacts the obtained values in female dogs.
  • Assay method selection is critical for accurate interpretation of progesterone levels during the periovulatory period.
  • Clinicians should be aware of potential discrepancies between RIA, CLIA, and ELISA when making reproductive management decisions in bitches.