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Pseudogene in the genome of bacteriophage lambda?

J Kypr, J Mrázek

    Biochemical and Biophysical Research Communications
    |May 29, 1987
    PubMed
    Summary

    Bacteriophage lambda

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    Area of Science:

    • Genomics
    • Molecular Biology
    • Bioinformatics

    Background:

    • The bacteriophage lambda genome contains non-coding regions with regulatory functions.
    • Specific DNA binding by proteins like repressors is crucial for viral gene regulation.

    Purpose of the Study:

    • To investigate a non-coding region in the bacteriophage lambda genome for potential protein-coding capacity.
    • To identify conserved DNA-binding motifs within this region.

    Main Methods:

    • Bioinformatic analysis of the bacteriophage lambda genome sequence.
    • Identification and analysis of open reading frames (ORFs).
    • Secondary structure prediction and codon usage analysis.

    Main Results:

    • A long ORF (>1kb) was identified in a non-coding region, read in the same frame as gene A.
    • The putative protein product exhibits a highly ordered secondary structure (alpha-helices), characteristic of DNA-binding proteins.
    • Codon usage suggests a protein-coding function, but a premature stop codon (TGA) was detected.

    Conclusions:

    • Bacteriophage lambda likely possessed an additional DNA-binding protein, possibly a repressor, in its evolutionary past.
    • This ancestral protein appears to have been inactivated by a mutation leading to the observed stop codon.

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