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Related Concept Videos

Spinal Cord01:26

Spinal Cord

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The spinal cord, a critical component of the central nervous system, extends from the base of the brainstem to the lumbar region of the vertebral column. It is essential for maintaining physical stability and facilitating communication between the brain and peripheral parts of the body.
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The Spinal Cord01:54

The Spinal Cord

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The spinal cord is the body’s major nerve tract of the central nervous system, communicating afferent sensory information from the periphery to the brain and efferent motor information from the brain to the body. The human spinal cord extends from the hole at the base of the skull, or foramen magnum, to the level of the first or second lumbar vertebra.
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Spinal Cord: Information Processing01:10

Spinal Cord: Information Processing

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The spinal cord is an integral hub for motor and sensory information that enables the brain to communicate with the peripheral nervous system (PNS). This communication consists of relaying sensory data and transmission of motor commands.
Sensory Information Processing
Sensory information processing begins at the sensory receptors located in the skin and other tissues, which detect somatic sensory stimuli such as touch, temperature, or pain. These receptors function as catalysts, initiating...
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Spinal Cord: Gross Anatomy01:15

Spinal Cord: Gross Anatomy

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The spinal cord resides within the protective confines of the vertebral column. It is the main pathway for information traveling between the brain and the body. It plays a fundamental role in nearly all bodily functions, from simple reflexes to complex motor movements. The spinal cord begins at the medulla oblongata at the base of the brainstem and extends downward, terminating at the conus medullaris near the first and second lumbar vertebrae. The spinal cord's length in adults is...
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Spinal Cord: Cross-sectional Anatomy01:16

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The cross-sectional anatomy of the spinal cord offers a detailed view of its complex structure and function within the central nervous system. At the core of the spinal cord lies the gray matter, characterized by its butterfly or "H"-shaped appearance in cross-section. This central region is enveloped by white matter, with the overall structure divided into symmetrical halves by the dorsal median sulcus and the ventral median fissure.
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¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

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A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
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Updated: Feb 13, 2026

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
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Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

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Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding.

Alexander B Rosenberg1, Charles M Roco2, Richard A Muscat3

  • 1Department of Electrical Engineering, University of Washington, Seattle, WA, USA. alex.b.rosenberg@gmail.com gseelig@uw.edu.

Science (New York, N.Y.)
|March 17, 2018
PubMed
Summary
This summary is machine-generated.

We developed split-pool ligation-based transcriptome sequencing (SPLiT-seq) for scalable single-cell RNA sequencing. This method identified over 100 cell types in developing mouse brains and spinal cords.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Neuroscience

Background:

  • Single-cell RNA sequencing (scRNA-seq) is crucial for understanding complex biological systems.
  • Existing scRNA-seq methods face challenges in scalability and compatibility with fixed samples.

Purpose of the Study:

  • To develop a scalable and versatile scRNA-seq method for comprehensive cellular profiling.
  • To analyze the transcriptomes of developing mouse central nervous system at single-cell resolution.

Main Methods:

  • Developed split-pool ligation-based transcriptome sequencing (SPLiT-seq) utilizing combinatorial barcoding.
  • Applied SPLiT-seq to analyze 156,049 single-nucleus transcriptomes from postnatal mouse brains and spinal cords.
  • Utilized pseudotime analysis to investigate developmental trajectories.

Main Results:

  • Identified over 100 distinct cell types based on gene expression patterns.
  • Characterized cell types by function, regional specificity, and differentiation stage.
  • Revealed transcriptional programs driving four major developmental lineages in the early postnatal murine CNS.

Conclusions:

  • SPLiT-seq enables scalable and efficient single-cell transcriptomic profiling.
  • Provides a detailed snapshot of early postnatal central nervous system development.
  • Offers a method for comprehensive analysis of other complex multicellular systems.