Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

81.7K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
81.7K
Membrane Proteins01:30

Membrane Proteins

30.6K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
30.6K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.7K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.7K
Sperm Transport01:15

Sperm Transport

3.6K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
3.6K
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

6.5K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.5K
GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

5.6K
GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sperm activation for fertilization requires robust activity of the TAT-5 lipid flippase.

Developmental biology·2025
Same author

The Caenorhabditis elegans spe-21 gene that encodes a palmitoyltransferase is necessary for spermiogenesis.

Developmental biology·2025
Same author

Sperm activation for fertilization requires robust activity of the TAT-5 lipid flippase.

bioRxiv : the preprint server for biology·2025
Same author

Utilizing <i>C. elegans</i> Spermatogenesis and Fertilization Mutants as a Model for Human Disease.

Journal of developmental biology·2025
Same author

Identification and sequencing of temperature sensitive alleles of the Anaphase Promoting Complex component <i>mat-3</i> in <i>C. elegans</i>.

microPublication biology·2023
Same author

The EGF-motif-containing protein SPE-36 is a secreted sperm protein required for fertilization in C. elegans.

Current biology : CB·2023

Related Experiment Video

Updated: Feb 13, 2026

Isolation and In vitro Activation of Caenorhabditis elegans Sperm
05:46

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Published on: January 31, 2011

17.0K

Caenorhabditis elegans sperm membrane protein interactome.

Matthew R Marcello1, Marina Druzhinina2,3, Andrew Singson2,3

  • 1Department of Biology, Pace University, New York, New York, USA.

Biology of Reproduction
|March 17, 2018
PubMed
Summary

Understanding sperm membrane protein interactions is key to reproductive health. This study mapped interactions in C. elegans, revealing novel connections crucial for sperm function and fertilization.

More Related Videos

Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.2K
Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
10:07

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Published on: June 6, 2019

13.0K

Related Experiment Videos

Last Updated: Feb 13, 2026

Isolation and In vitro Activation of Caenorhabditis elegans Sperm
05:46

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Published on: January 31, 2011

17.0K
Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.2K
Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
10:07

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Published on: June 6, 2019

13.0K

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Genetics

Background:

  • Sperm membrane protein organization is vital for sperm function, including fertilization.
  • Defects in sperm function can arise from altered protein interactions.
  • The nematode Caenorhabditis elegans serves as a model for studying reproductive processes.

Purpose of the Study:

  • To identify and characterize interactions among known sperm membrane proteins.
  • To understand the molecular basis of sperm function and identify potential causes of infertility.
  • To investigate the roles of specific proteins in spermatogenesis, spermiogenesis, and fertilization.

Main Methods:

  • Utilized a split-ubiquitin membrane yeast two-hybrid system.
  • Analyzed 12 mutationally defined and cloned sperm membrane proteins.
  • Focused on gene products essential for sperm function and predicted to be transmembrane proteins.

Main Results:

  • Identified novel interactions between sperm membrane proteins.
  • Revealed that SPE-38, a protein involved in fertilization, interacts with proteins essential for spermatogenesis and spermiogenesis.
  • Suggested SPE-38 may act as a central organizing protein within the sperm plasma membrane.

Conclusions:

  • Novel protein interaction pairings provide a foundation for further research into sperm membrane organization.
  • Understanding these interactions can lead to better characterization of sperm function defects.
  • This work enhances the understanding of molecular mechanisms underlying successful fertilization.