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Updated: Feb 13, 2026

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
Published on: September 8, 2016
Isolation of Schwann Cell Precursors from Rodents
Rhona Mirsky1, Kristjan R Jessen2
1Department of Cell and Developmental Biology, University College London, London, UK.
Abstract:
Schwann cell precursors are the first defined stage in the generation of Schwann cells from the neural crest and represent the glial cell of embryonic nerves. Highly pure cultures of these cells can be obtained by enzymatic dissociation of nerves dissected from the limbs of 14- or 12-day-old rat and mouse embryos, respectively. Since Schwann cell precursors, unlike Schwann cells, are acutely dependent on axonal signals for survival, they require addition of trophic factors, typically β-neuregulin-1, for maintenance in cell culture. Under these conditions they convert to Schwann cells on schedule, within about 4 days.
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