Measurement of refractive index of hemoglobin in the visible/NIR spectral range
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Updated: Feb 13, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
Published on: January 10, 2025
Ekaterina N Lazareva1,2, Valery V Tuchin1,3,4
1Saratov State University (National Research University), Research Educational Institute of Optics an, Russia.
Abstract:
This study is focused on the measurements of the refractive index of hemoglobin solutions in the visible/near-infrared (NIR) spectral range at room temperature for characteristic laser wavelengths: 480, 486, 546, 589, 644, 656, 680, 930, 1100, 1300, and 1550 nm. Measurements were performed using the multiwavelength Abbe refractometer. Aqua hemoglobin solutions of different concentrations obtained from human whole blood were investigated. The specific increment of refractive index on hemoglobin concentration and the Sellmeier coefficients were calculated.
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