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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy
1CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety Institute of High Energy Physics, Chinese Academy of Sciences 19B, Yu Quan Road, Beijing 100049, China. limin@ihep.ac.cn and Institute of Physical Science and Information Technology, Anhui University, Anhui, 230601, China.
Abstract:
A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I1574/I1586 for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.
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