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Updated: Feb 13, 2026

The Hawaii Protocol for Scientific Monitoring of Coffee Berry Borer: a Model for Coffee Agroecosystems Worldwide
Published on: March 19, 2018
Rapid sucrose monitoring in green coffee samples using multienzymatic biosensor
Miroslav Stredansky1, Luca Redivo2, Peter Magdolen3
1Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, 81237 Bratislava, Slovak Republic; Biorealis s.r.o., Radlinskeho 9, 81107 Bratislava, Slovak Republic.
Abstract:
Amperometric biosensor utilizing FAD-dependent glucose dehydrogenase (FAD-GDH) for a specific sucrose monitoring in green coffee is described. FAD-GDH was co-immobilized with invertase and mutarotase on a thin-layer gold planar electrode using chitosan. The biosensor showed a wide linearity (from 10 to 1200 μM), low detection limit (8.4 μM), fast response time (50 s), and appeared to be O2 independent. In addition the biosensors exhibited a good operational (3 days) and storage (1 year) stability. Finally, the results achieved from the biosensor measurements of sucrose in 17 samples of green coffee (Coffea arabica, C. canephora and C. liberica) were compared with those obtained by the standard HPLC method. The good correlation among results of real samples, satisfactory analytical performance and simple use of the presented biosensor make it suitable for application in coffee industry.
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